148 Report of the Department of Bacteriology of the 



The amount of blood in the milk from the right front quarter 

 was less on the next morning (March 5) but again appeared in 

 quantity at the evening milking. No blood showed in the milk 

 from the other quarters at this milking. The milk from the right 

 front quarter was still slightly bloody on March 6 but no blood was 

 noticeable after this date. The udder was distended and inflamed 

 for several days but was not noticeably indurated. In other words, 

 the changes in the udder and milk were not especially unusual 

 except in the fact that the right front quarter discharged rather 

 more blood than normally occurs. 



A study of the cell content of the milk of this animal as given 

 in Graph I (continuous line) shows that the greatest number 

 of cells occurred in the first milk drawn after calving and that there 

 was a very rapid diminution in number the following seven days 

 until the low figure of 35,000 cells per cubic centimeter was found. 

 On the following day there was a sudden return to a maximum of 

 4,050,000 cells per cubic centimeter, a figure which approaches 

 those of the first two days. This was followed by a falling off in 

 numbers during the following three days with another return to a 

 maximum of 4,350,000 cells per cubic centimeter on March 14. 

 The cell counts were low through the remainder of the period during 

 which Chloe B was under observation, and none of the counts obtained 

 later from her as given in Table II (p. 132) are excessively high. 



A study of the counts from the individual quarters explains the 

 details of some of these fluctuations. The right front quarter 

 which gave the bloody milk on March 4 to 6 shows higher counts 

 than the other quarters on March 4 and 5 but not on March 6 and 

 at no time are the cell counts as much higher as would have been 

 expected from the appearance of the milk. The count of 130,000 

 cells per cubic centimeter for the right front quarter on the eve 

 of March 6, when the milk from this quarter was still noticeably 

 bloody, is a low count and shows at once the impossibility of detecting 

 bloody milk when mixed with herd milk by means of the cell count 

 alone. After the first few days the number of cells in the milk 

 of this quarter was very low and continued low as long as the milk 

 was tested. 



In none of the smears prepared from bloody milk were the red 

 blood cells counted as they do not show clearly when the smears 

 are stained with methylene blue as was done in this case. 



