New York Agricultural Experiment Station. 159 



average number of cells in her milk than the machine-milked cows 

 is probably of no significance. 



It is difficult to see how the abnormality noted in the case of 

 Mabel S. F. could cause an increase in the number of cells dis- 

 charged and it is probable that the two things were not related 

 to each other in any way. Since the average number of cells dis- 

 charged (705,000 per cubic centimeter) is not as high as the average 

 number of cells given for all of the cows examined (868,000 per 

 cubic centimeter) and is not decidedly higher than the average 

 number found in the case of other apparently normal cows in the 

 same herd (see Table III), there is little reason for regarding the 

 discharge of this number of cells as abnormal. Moreover Wilson 

 did not note anything especially interesting in the bacterial flora 

 of the udder of this cow in several examinations which he made 

 during the previous summer. (See Technical Bulletin 27, Table X.) 

 The predominant organism was the same as the one which has already 

 been mentioned in connection with Chloe B. (page 151) and Ruth 

 F. B. B. (page 153) which had the group number M. 211.2233033. 

 None of the tests made in March, April or May, 1910, showed bac- 

 terial counts higher than 176 per cubic centimeter. Among the 

 July, 1910, tests, one showed an average of 1100 per cubic centimeter 

 for the left front quarter of this cow. The other quarters all gave 

 low counts. 



In the case of Millie G. there is reason for believing that there 

 was a relation between the relatively high cell count (958,000 per 

 cubic centimeter) and her previous udder trouble. No tests were 

 made at this time to determine whether the high cell count was 

 caused by an excessive discharge of cells from the quarter which 

 had been most seriously affected (left hind), but it is quite possible 

 that this was the case. Table X in Technical Bulletin No. 27 

 shows the organisms which Wilson isolated from this cow's udder. 

 None of these were streptococci but a study of his notes shows 

 that he failed to isolate the predominating organism present in 

 all but the left hind quarter. This organism v/as present in large 

 numbers, that is the bacterial counts frequently exceeded 1,000 

 per cubic centimeter, and sometimes showed such large numbers 

 of colonies on the plate that they could not be counted. The 

 colonies were small, gave a sour smell to the plates and appeared 

 in practically pure culture. A culture isolated from the right hind 



