238 Report of the Department of Botany of the 



night. The remaining plants were heeled in out-of-doors until the 

 first of February, when they were all transplanted beside the others. 



"All the infected plants picked up very rapidly, developing good 

 foliage, which remained green until December, 1913, or for more 

 than one year, when most of them were taken out and thrown away. 

 None had showed any indications of rusting during this time, and 

 the few remaining bushes are free from rust at the present time 

 (February, 1914). The plants which were put into the houses first 

 without being heeled in were used in most cases for fumigation 

 experiments. 



"Careful inspection of the estate in Ipswich in 1913 showed that 

 both the black and red currants were infected, the red currants 

 suffering less than the others. Most of them were interplanted 

 with apple trees and covered about thirty acres in blocks not far 

 distant from one another. During the fall of 1912 inspection was 

 made of the neighboring pines to see whether there was any rust, 

 but none was noted. The past year, however, a number of pines 

 near the infected currants have shown the rust, but this infection 

 did not extend more than 100 yards from the currants. All of this 

 infection is confined to imported white pines, no trace of infection 

 being found on the native white pine. During 1913 six new loca- 

 tions, all in the eastern part of the state, were discovered for the 

 currant rust, and in all thirty new pine infections. The state inspec- 

 tors are doing everything possible to destroy infected material and 

 establish a careful quarantine." 



The 100 plants sent to Ithaca in the late autumn were heeled 

 in out-of-doors until the second week of January when they were 

 set out in benches in the greenhouse. They soon leafed out and 

 grew vigorously. Careful observations were made for infections 

 but none occurred. No attempt was made to inoculate the plants 

 with wintered leaves. In May, after the finding of the two affected 

 white pines at Geneva, inoculations were made by dusting the aecio- 

 spores from this material onto moistened Ribes leaves. The inocu- 

 lated plants were covered with bell-jars and kept shaded for two 

 days. Abundant infection resulted and the uredinia and telia were 

 developed on almost every leaf of the 100 plants. 



In the experiment at Geneva, 100 of the yearling black currant 

 plants were placed in the greenhouse on December 2, but they did 



