]STew York Agricultural Experiment Station. 275 



from the cut. Some cases were found where there was no apparent 

 effect. 



On the date of the third examination, July 3, 1912, three of the 

 check vines showed evidence of disease, one apparently affected 

 before the cuts were made. The lesions on the other two were 

 well up in the vine and were removed in pruning. 



Of the inoculated vines fifteen showed no apparent effect of the 

 inoculation. Eleven of the remainder showed symptoms of dead- 

 arm disease, some apparently due to the inoculations. One vine 

 was dead and removed and another very sickly but probably not from 

 inoculation. 



July 10, 1913, five of the vines serving as checks were in good 

 condition. Of the inoculated vines eleven were recorded as in good 

 condition. Four others seemed in fair condition while the remainder 

 were nearly dead or in very poor condition. The vines were cut 

 off near the ground October 22, 1913, and shipped to Ithaca. October 

 29th, the inoculated vines were split open and many were found to 

 be decayed. In some cases the discoloration extended over half 

 way through the stem and six inches or more up and down the stem 

 from the original point of inoculation. Attempts were made to 

 remove bits of the discolored tissue under aseptic conditions and 

 transfer them to sterilized grape stems. Of twenty-four such transfers 

 made, nine developed pure cultures of the fungus, ten were con- 

 taminated so that it could not be determined whether Cryptosporella 

 was present or not and five remained sterile. It appears from this 

 set of inoculations that infection may occur from the pruning saw, 

 that the fungus does not spread rapidly in the tissue and that dis- 

 coloration of the woody tissue precedes the advance of the fungus. 

 Examination of the checks failed to reveal the typical discolora- 

 tion of the dead-arm disease. Some showed only a blackening of the 

 wood as from weathering and others showed the lighter brown decay 

 characteristic of the action of the fungus Pohjstictus versicolor. This 

 only extended a short distance from the original cut. 



Second series. — A series of inoculations was made June 22, 1909, 

 in which spores and mycelium of the fungus from a pure culture 

 (3221 isolated May 9, 1909, from exuding spores on 1-year cane) 

 in its second generation were inserted in the cuts. The record for 

 the thirty vines thus inoculated does not differ materially from that 

 above except that most of the vines showed more striking symptoms 



