New York Agricultural Experiment Station. 277 



one dead canes were counted on the vines of this row. Although 

 the vines were partial ly renewed by tying up canes back of the 

 point of inoculation the row was conspicuous on account of dead 

 canes from the infected arms. 



The same organism was inoculated into the canes of twenty- 

 three other Worden vines on July 9, 1909. The photograph shown 

 in Fig. 14, is one of these inoculated canes after fourteen months. 

 Two dead arms were found in 1912, but in general the symptoms 

 were not nearly so marked as in the set of inoculations on shoots. 



A culture from a different source (3261) was used for inoculating 

 a number of bearing shoots July 8, 1910. There was no noticeable 

 effect in 1911, but in 1912 a number of arms showed marked symptoms 

 and on one pycnospores were oozing in abundance. In July, 1913, 

 there were a number of dead stubs and canes on the five vines 

 inoculated. 



INOCULATION OF ROOTS. 



July 18, 1910, the soil was dug away from one side of seven Worden 

 vines and several roots, both large and small, were inoculated through 

 a scalpel wound near the root crown with mycelium and spores of the 

 fungus (3261). 



Observations from year to year failed to show any marked effect. 

 In the autumn of 1913, the vines were pulled up and shipped to 

 Ithaca without label. A careful examination was made of all the 

 roots, but the original point of inoculation could not be located 

 with certainty except in one instance. All the roots were examined, 

 but in no case was there any evidence of disease. This bears out 

 frequent observations that the diseased area does not as a rule 

 extend below the surface of the ground, and is a point of consider- 

 able importance in control. 



BIBLIOGRAPHY. 

 Bubak, Fr., u. Kosaroff, P. Einige interessante Pnanzen-krankheiten aus Bulgaria. 



Centbl. Bakt. Abt. II, 31:495-502. 1911. 

 Cavara, F. Intorno alia eziologia di alcufie malattie di piante coltivate. Stiz- 



Sper. Agr. ltd. 30:487. 1897. 

 Gregory, C. T. A rot of grapes caused by Cryptosporella viticola. Phytopath. 



3:20-23. Figs. 1-2. 1913. 

 Reddick, D. Necrosis of the grape vine. Cornell Univ. Agr. Exp. Sta. Bui. 



263:323-343. Figs. 41-57. 1909. 



