324 Report of the Department of Chemistry of the 



1 to 1.8 per ct. lower than required for inosite hexaphosphate. As 

 will be shown later in this bulletin, this is due to the fact that 

 the free acid becomes largely hydrolyzed on drying. 



EXPERIMENTAL PART. 

 ISOLATION AND PURIFICATION OF THE BARIUM SALT. 



The cottonseed meal, 25 pounds, was digested over night in 

 0.2 per ct. hydrochloric acid in porcelain percolators covered on the 

 inside with a double layer of cheesecloth. It was then percolated 

 using 0.2 per ct. hydrochloric acid until about 20 liters of extract 

 were obtained. The extract was of a dirty, dark color and contained 

 some suspended particles from which it was freed as much as possible 

 by centrifugalizing the solution. A concentrated solution of 300 

 grams of barium chloride was then added and the precipitate 

 allowed to settle. The precipitate was centrifugalized and finally 

 brought upon a Buchner funnel and freed as far as possible from the 

 mother-liquor. It was then digested in several liters of about 5 

 per ct. hydrochloric acid until no further solution took place. 

 The insoluble residue was removed by centrifugalizing and the still 

 very dirty colored solution precipitated by adding barium hydroxide 

 until the free acid was neutralized. The barium hydroxide was 

 added slowly, with constant shaking, when the precipitate separated 

 in crystalline form. It was then filtered and washed thoroughly 

 in water and again dissolved in dilute hydrochloric acid, filtered 

 and reprecipitated with barium hydroxide. These operations 

 were repeated three times. The hydrochloric acid solution was then 

 precipitated by gradually adding an equal volume of alcohol when 

 the substance again separated in crystalline form consisting of 

 globular masses of microscopic needles. It was then precipitated 

 a fourth time with barium hydroxide and after that two more times 

 with alcohol. It was then filtered, washed free of chlorides with 

 dilute alcohol and then in alcohol and ether and dried in vacuum 

 over sulphuric acid. The product was then a nearly white, crys- 

 talline powder and it weighed 94 grams. 



The dry substance was shaken up with about 1.5 liters of cold 

 water, allowed to stand for several hours and then filtered and 

 washed in water. The aqueous solution contained very little sub- 

 stance precipitable with alcohol and it was therefore discarded. 



The washed precipitate was dissolved in dilute hydrochloric 

 acid and precipitated a fifth time by the very gradual addition of 

 barium hydroxide; after filtering and washing, this operation was 

 repeated a sixth time. After again dissolving in dilute hydrochloric 

 acid, nearly neutralizing the free acid with barium hydroxide and 

 filtering, the substance was brought to crystallization by the gradual 

 addition of an equal volume of alcohol. After standing for several 

 hours the substance was filtered and washed in dilute alcohol, 



