348 Report ok the Department of Chemistry of the 



was filtered and washed thoroughly in water. It was dissolved 

 in the minimum quantity of about 3 per ct. hydrochloric acid, 

 filtered and again precipitated with barium hydroxide. These 

 operations were repeated four times. The substance was then 

 precipitated from the same strength hydrochloric acid with alcohol. 

 After thoroughly washing the precipitate with dilute alcohol it 

 was again dissolved in 3 per ct. hydrochloric acid and precipi- 

 tated a fifth time with barium hydroxide. The dilute hydro- 

 chloric acid solution of the substance was then twice precipitated 

 with alcohol. After finally filtering, the precipitate was washed 

 free of chlorides with dilute alcohol and then washed in alcohol and 

 ether and dried in vacuum over sulphuric acid. The substance 

 was then a snow-white amorphous powder. The dry powder was 

 rubbed up in a mortar with a small quantity of cold water. The 

 insoluble portion changed into a semi-crystalline form after a short 

 time. This was filtered and washed thoroughly in water. It was 

 dissolved in the minimum quantity of 3 per ct. hydrochloric acid. 

 A dilute solution of barium hydroxide was then added until a slight 

 permanent precipitate remained which was nearly cleared up by 

 the careful addition of dilute hydrochloric acid. The solution was 

 filtered and allowed to stand over night. The substance soon 

 began to separate in crystalline form. Under the microscope it 

 appeared perfectly homogeneous and the crystal form was identical 

 with that observed with the barium salts from cottonseed meal, 

 oats and corn, i. e. the substance crystallized in globular masses of 

 microscopic needles. 



The substance was filtered off and washed free of chlorides with 

 water and then in alcohol and ether and dried in vacuum over 

 sulphuric acid. 



To the mother-liquor a concentrated solution of 15 grams of barium 

 chloride was added and allowed to stand for another 24 hours. 

 A further quantity of the same-shaped crystals had then separated 

 which were filtered, washed and dried as above. 



The two crystalline portions were united and recrystallized in 

 the same manner and again dried in vacuum over sulphuric acid. 

 It was then dissolved in the same strength hydrochloric acid and 

 precipitated by adding an equal volume of alcohol. The precipitate 

 was amorphous at first but after standing a few hours it had changed 

 into the crystalline form — identical with the above but the crystals 

 were much smaller. After standing over night it was filtered, washed 

 free of chlorides with dilute alcohol and then in alcohol and ether 

 and dried in vacuum over sulphuric acid. 



The product was a snow-white, light, bulky crystalline powder. 

 It weighed 24 grams. It was free from chlorides and inorganic 

 phosphate. In 0.5 gram of the substance no bases other than barium 

 could be detected. 



