35G Report of the Department of Chemistry of the 



PURIFICATION OF THE BARIUM AND PHOSPHORUS CONTAINING PRECIPI- 

 TATE REMOVED FROM THE INOSITE SOLUTION WITH ALCOHOL. 



The substance mentioned above, precipitated with alcohol, was 

 apparently the barium salt of an organic phosphoric acid but it 

 differed in solubility from any other salt of this nature previously 

 observed. It was very soluble in water and was not precipitated 

 from the aqueous solution by barium hydroxide. The dry sub- 

 stance weighed 1.2 grams. It was dissolved in a small quantity 

 of water, a few drops of dilute hydrochloric acid added and 10 cubic 

 centimeters of N /i barium chloride. The solution was heated to 

 boiling and alcohol added until a slight cloudiness was produced. On 

 standing in the cold over night a small amount of a hard crust had 

 separated on the bottom of the flask. This was removed and the 

 solution again heated and more alcohol added when a further 

 quantity separated in the same way. The substance was finally 

 filtered and washed thoroughly in 80 per ct. alcohol, alcohol and 

 ether and dried in the air. Without further purification the sub- 

 stance was analyzed after drying at 105° in vacuum over phosphorus 

 pentoxide. 



Found: C = 20.03; H = 3.58; P = 8.53; Ba = 25.55 per ct. 



The small quantity precluded recrystallization and we are there- 

 fore unable to state whether the substance was pure. The analytical 

 result indicates that it was a barium salt of inosite monophosphate 

 and agrees approximately with this formula: 



C 18 H 35 O27P 3 Ba 2 = 1051. 



Calculated: C = 20.55; H = 3.33; P = 8.84; Ba = 26.16 per ct. 



Such a salt could be represented by the following formula: 

 CeHuO, - P E? Q H 



C 6 H n 6 — P=0 ( 



>Ba 



O 



>Ba 



CeHnOe — P=0 0H 



EXAMINATION OF THE PRECIPITATE PRODUCED WITH BARIUM 

 HYDROXIDE AFTER DRYING THE ABOVE ACID. 



A portion of the barium precipitate was dried in vacuum over 

 sulphuric acid and then examined for total and inorganic phosphorus 

 in the same way as before: 



Found : Total phosphorus (by Neumann method) 9.98 per ct. 

 Found: Inorganic phosphorus 7.46 per ct. 



