New York Agricultural Experiment Station. 357 



As will be noticed from these figures 74.76 per ct. of the phos- 

 phorus was present as inorganic phosphoric acid. 



The substance was freed from inorganic phosphate by precipitating 

 four times with alcohol from 0.5 per ct. hydrochloric acid. After 

 finally drying in vacuum over sulphuric acid 3.2 grams of a snow- 

 white, amorphous powder was obtained. The substance was free 

 from chlorides and inorganic phosphate. 



It was shaken up with about 75 cubic centimeters of cold water 

 in which the greater portion dissolved; 10 cubic centimeters of N /i 

 barium chloride was added and allowed to stand for several hours; 

 the insoluble portion was then filtered off, washed free of chlorides 

 with water and then in alcohol and ether and dried in vacuum 

 over sulphuric acid. It weighed 0.65 gram. 



The filtrate from above containing the water-soluble portion of 

 the substance was acidified with a few drops of dilute hydrochloric 

 acid, heated to boiling and alcohol added until a slight permanent 

 cloudiness remained. On standing over night, a portion had sepa- 

 rated in the form of a heavy, granular powder. Under the micro- 

 scope no definite crystal form could be observed but it appeared to 

 consist of transparent globules. It was filtered off, washed free of 

 chlorides in 30 per ct. alcohol, alcohol and ether and dried in the 

 air. Yield 0.67 gram. It was free from inorganic phosphate. 



The mother-liquor from above was precipitated with alcohol. 

 After settling, the precipitate was filtered, washed with dilute alcohol, 

 alcohol and ether and dried in vacuum over sulphuric acid. Yield 

 1.55 grams. The substance was a snow-white, amorphous powder. 

 It was free from chlorides and inorganic phosphate. 



These three different portions were analyzed after drying at 105° 

 in vacuum over phosphorus pentoxide. 



The water-insoluble portion gave 



C == 9.40; H = 1.65; P = 13.76; Ba = 39.56 per ct. 



Judging by the analysis this substance consists mainly of the 

 barium salt of inosite tetraphosphate. 



The granular powder which separated from the hot dilute hydro- 

 chloric acid solution and alcohol on cooling gave the following 

 result : 



C = 12.70; H = 2.40; P = 13.84; Ba = 32.29; H 2 = 12.77 

 per ct. 



This substance appears to be mainly the barium salt of inosite 

 triphosphate, although not pure. It was mixed probably with some 

 barium salt of inosite diphosphate. 



The water-soluble portion precipitated with alcohol gave: 



C = 14.07; H = 2.31; P = 12.92; Ba = 33.04 per ct. 



