372 Report of the Department of Chemistry of the 



The barium precipitate was free from chlorides and inorganic 

 phosphate. It was analyzed after drying at 105° in vacuum over 

 phosphorus pentoxide. Found: C = 11.66; H = 2.11; P = 14.14; 

 Ba = 34.36 per ct. 



This substance is therefore identical in composition with the first 

 preparation reported above and agrees very closely with the com- 

 pounds previously described. 



It is clearly evident from the results recorded above that an acid, 

 C12H41O42P9, such as described by Rather, does not exist, at least 

 not in wheat bran or cottonseed meal. The alleged, " pure, homo- 

 geneous silver salts," analyzed by the above author must have been 

 largely contaminated with silver phosphate and this simple impurity 

 escaped his observation. 



FURTHER PREPARATIONS OF THE BARIUM SALTS OF THE ORGANIC 

 PHOSPHORUS COMPOUND OF WHEAT BRAN. 



The 0.2 per ct. hydrochloric acid extract of wheat bran was 

 precipitated by adding a concentrated solution of barium chloride. 

 After settling, the precipitate was filtered and washed in dilute 

 alcohol, alcohol and ether and dried in vacuum over sulphuric acid. 



To the mother-liquor from above, barium acetate was added 

 which caused a further precipitate. This was filtered, washed and 

 dried in vacuum over sulphuric acid. 



Total and inorganic phosphorus were determined in these precipi- 

 tates as follows: Total phosphorus was determined after decom- 

 posing by the Neumann method. As inorganic phosphorus we 

 consider the amount of phosphorus directly precipitated by ammo- 

 nium molybdate from the nitric acid solution of the substance at 

 a temperature of 65° for one-half hour. 



Found in the precipitate produced with barium chloride: 



Total phosphorus = 8.45 per ct. 



Inorganic phosphorus = 1.55 per ct. 



The result shows that 18.32 per ct. of the total phosphorus was 

 present as inorganic. 



Found in the precipitate produced with barium acetate: 



Total phosphorus = 10.28 per ct. 



Inorganic phosphorus = 8.63 per ct. 



In this case 83.96 per ct. of the total phosphorus was present as 

 inorganic. 



The above barium precipitates were purified separately in the 

 same way as before by repeatedly precipitating with barium hy- 

 droxide and alcohol alternately from 0.5 per ct. hydrochloric acid 

 until pure white amorphous powders were obtained which were 

 free from inorganic phosphate and which contained no bases except 

 barium. 



After drying at 105° in vacuum over phosphorus pentoxide the 

 following results were obtained on analysis: 



