New York Agricultural Experiment Station. 373 



The preparation isolated from the barium chloride precipitate: 

 Found: C = 11.53; H = 2.10; P = 14.29; Ba = 34.60 per ct. 



This substance has the same composition as the precipitates 

 obtained from the previously isolated crude acid. These various 

 preparations were therefore mixed and treated as will be described 

 later. 



The preparation isolated from the barium acetate precipitate: 

 Found: C - 15.22; H = 2.62; P = 12.22; Ba = 29.85 per ct. 



Several other preparations were made from wheat bran in differ- 

 ent ways. The composition varied considerably, however, as is 

 evident from the figures given below. 



One preparation gave the following: 



C = 15.33; H = 2.70; P = 11.61 ; Ba = 32.31 per ct. 



Another gave: 



C = 14.62; H = 2.65; P = 12.84; Ba = 30.59 per ct. 



A third preparation gave: 



C = 13.94; H = 2.47; P = 13.10; Ba = 31.41 per ct. 



A fourth gave: 



C = 15.01 ; H = 2.67; P = 12.25; Ba = 30.94 per ct. 



treatment of the amorphous barium salt with cold water. 



The barium precipitates obtained from the crude acid as well 

 as the substance isolated from the barium chloride precipitate 

 which, as shown above, had the same composition, were mixed 

 together. Total weight 22.5 grams. It was rubbed up in a mortar 

 with 200 c.c. of cold water when the greater portion dissolved. The 

 insoluble substance was filtered, washed in water, alcohol and ether 

 and dried in vacuum over sulphuric acid. It was then treated with 

 a second portion of 200 c.c. of cold water, again filtered, washed and 

 dried. This insoluble residue weighed 5.75 grams. It had the 

 following composition after drying at 105° as before: 



Found: C = 9.52; H = 1.53; P = 12.89; Ba = 41.79 per ct. 



The filtrate from the above, containing the water-soluble portion 

 of the barium salt, was heated to boiling. The solution turned 

 first cloudy but gradually a white flocculent precipitate separated 

 which soon assumed a granular form and settled to the bottom of 

 the flask. This was filtered, washed in boiling water, alcohol and 

 ether and dried in the air. Under the microscope the substance 

 showed no definite crystalline structure but consisted of fine trans- 

 parent globules. It weighed 1.6 grams. It was free from inorganic 

 phosphate. 



It was analyzed after drying at 105° in vacuum over phosphorus 

 pentoxide. 



Found : C = 9. 15 ; H = 1 .79 ; P = 13.57 ; Ba = 40.43 ; H 2 = 9.53 per ct. 

 C - 8.96; H = 1.62. 



