New York Agricultural Experiment Station. 375 



Another preparation gave the following: 



C = 11.26; H = 1.58; P - 10.67; Ba = 37.81; H 2 = 12.00 per ct. 



Judging by these results it is evident that wheat bran contains 

 more than one organic phosphoric acid. It appears probable that 

 several are present and that the solubility of the salts of such acids 

 differs so slightly that their separation is very difficult. Until defi- 

 nitely homogeneous products can be separated from this mixture it 

 seems futile to develop empirical formulas; for such may be calcu- 

 lated for every substance analyzed. The investigation is being 

 continued. 



The author wishes to express his appreciation and thanks to Dr. 

 P. A. Levene of the Rockefeller Institute for Medical Research, 

 New York, N. Y., and to Dr. Thomas B. Osborne of the Connecticut 

 Agricultural Experiment Station, New Haven, Conn., for many 

 valuable suggestions. 



II. CONCERNING INOSITE MONOPHOSPHATE, A NEW 

 ORGANIC PHOSPHORIC ACID OCCURRING IN 



WHEAT BRAN.* 



(Tenth Paper on Phytin.) 



INTRODUCTION. 



In previous reports 1 we have shown that the crude organic phos- 

 phorus compound of wheat bran 2 can be separated into two portions 

 by treating it with barium hydroxide. The insoluble precipitate 

 which forms under these conditions contains the barium salts of 

 certain not yet identified organic phosphoric acids and it is free from 

 nitrogen. By evaporating the filtrate from the above insoluble 

 barium salts a substance is obtained which is rich in nitrogen and 

 which also contains phosphorus in organic combination. 



In the further investigation of this soluble nitrogen-containing 

 substance it was found that its aqueous solution gave an insoluble 

 precipitate with lead acetate. The only other salt which gave any 

 precipitate was copper acetate and then only on warming when a 



* The work reported in this paper was carried out in the I. Chem. Institut der 

 Universitat zu Berlin, Berlin, Germany. 



iJour. Biol. Chem. 12:447 (1912); N. Y. Agric. Exp. Sta. Tech. Bull. 22 (1912), 

 and also the preceding article. 



2 This crude compound had been prepared by precipitating the 0.2 per ct. hydro- 

 chloric acid extract of wheat bran with alcohol. The resulting precipitate was then 

 purified by repeatedly precipitating from 0.2 per ct. hydrochloric acid with alcohol 

 until a nearly white product was obtained which was easily soluble in cold water and 

 which gave no precipitate with ammonium molybdate. Concerning its preparation, 

 see the above publications. 



