New York Agricultural Experiment Station. 379 



excess of barium hydroxide removed with carbon dioxide. The 

 filtrate was evaporated to dryness on the water-bath. The residue 

 gave no precipitate with ammonium molybdate, but after decom- 

 posing by the Neumann method, a heavy precipitate of ammonium 

 phosphomolybdate was obtained showing that only a portion of 

 the acid had been hydrolyzed under the above conditions. The 

 residue, however, contained some inosite which was isolated as 

 follows : The substance was taken up in a few cubic centimeters of 

 hot water, a little more than an equal volume of alcohol was added 

 which caused a voluminous white amorphous precipitate consisting 

 of the barium salt of the unchanged inosite monophosphate. After 

 filtering, the precipitate was again treated with water, again precipi- 

 tated with alcohol and filtered. The filtrates were evaporated on 

 the water-bath, taken up in a little water and the inosite brought 

 to crystallization by the addition of alcohol and ether. It crystallized 

 in the usual needle-shaped crystals. After standing several hours 

 in the ice chest the crystals were filtered, washed in alcohol and ether 

 and dried in the air. Yield 0.06 gram. It gave the reaction of 

 Scherer and melted at 224° C. (uncorrected). 



II. ALKALINE HYDROLYSIS. 



Another portion of the acid, 0.4 gram, was heated in a sealed 

 tube with 10 c.c. of 10 per ct. ammonia for six hours to 120°. The 

 solution then contained some free phosphoric acid as it gave a 

 precipitate with ammonium molybdate but the greater portion of 

 the acid remained unchanged. It was found impossible to isolate 

 any inosite from this reaction mixture. 



The residue was therefore again heated in a sealed tube with 10 

 per ct. ammonia for about 4| hours to 150°. In this case complete 

 hydrolysis had taken place, and after isolating the inosite in the 

 usual way 0.15 gram was obtained. This was recrystallized three 

 times from dilute alcohol with addition of ether and was then 

 obtained in colorless needles free from water of crystallization. It 

 then melted at 224° C. (uncorrected), and it gave the reaction of 

 Scherer. The identity of the substance was further confirmed by 

 the analysis. 



0.1206 gram subst. gave 0.0755 gm. H 2 and 0.1761 gm. C0 2 . 



Found : C = 39.82 ; H = 6.97 per ct. 



For C 6 H 12 6 = 180. 



Calculated : C = 40.00; H = 6.66 per ct. 



The author wishes to express his appreciation and thanks to His 

 Excellency Prof. E. Fischer and to Prof. H. Leuchs for the kind 

 interest which they have shown in the work reported in this paper. 



