!N"ew Yoek Agkicultukal ExPEi?T7irEXT Station. 335 



bark, by taking small particles from the inner bark with sterile 

 instruments. Two forms of fungi appeared in these cultures 

 more or less constantly, which led to their being separated and 

 transferred to sterilized bean stems in test tubes. Here they 

 grew luxuriantly and soon produced fruit, the one form produc- 

 ing the familiar dark colored spores which were not at that time 

 identified, while in the other the sporophores of Schizophyllum 

 commune Fr. were formed. 



Inoculations were made with the cultures on June 22 on seed- 

 ing apple trees in the nursery row as follows: Three trees were 

 inoculated with material from cultures of the dark spored fungus, 

 three trees with material from cultures of SchizophyUum com- 

 mune, and three trees were punctured but not inoculated to serve 

 as checks. The inoculations were made by cutting a small open- 

 ing in the bark with a sterilized knife and inserting a small 

 amount of the material from the bean stem cultures between the 

 bark and wood. All of the punctures were covered mth filter 

 paper which was kept moist for about thirty-six hours. On the 

 same date two inoculations with each of the two cultures, together 

 with check wounds, were made in the larger limbs of a mature 

 apple tree. These inoculations were not moistened or protected 

 in any way. In two weeks' time there was an area of discolored 

 bark around each place of inoculation where the unknown fungus 

 had been inserted. The other inoculations as well as the checks 

 showed no signs of growth and the wounds soon healed. 



As soon as it was known that the one fungus could penetrate 

 living bark under certain conditions more inoculations were made. 

 July 6, six young seedling apple trees in the nursery row^ and 

 three limbs of a large apple tree were inoculated witb the dark 

 spored fungus, six seedling nursery trees and three limbs of 

 a larger tree with SchizophyUum commune, while three seedling 

 nursery trees and three limbs of a large tree were punctured but 

 not inoculated to serve as checks. The inoculations made in the 

 seedling trees were all protected with filter paper as before, but 



