Xew York Agkicultuual Experiment Station. 365 



which had held the cluster which furnished the pollen. After the 

 bag had been closed and labeled, it was shaken so as to agitate the 

 loose pollen which it might contain and possibly assist in more 

 thoroughly distributing it among the open flowers of the cluster 

 which was to be pollinated. 



In the Penn Yan tests and in those at the Station, in addition 

 to the treatment above outlined, the open flowers of the cluster 

 which were selected to supply the pollen were brushed over the 

 open flowers which were to be cross-pollinated before fastening the 

 two clusters together. With most varieties the interlocking of the 

 branches of the two clusters was sufficient to hold the loose cluster 

 in place, but in some instances it was necessary to tie the two to- 

 gether. Judging from their appearance Avhen the bags were 

 opened after the blooming season had passed, the flowers of the 

 detached clusters which were not open when the bag was finally 

 closed and labeled, generally failed to open later. It appears, 

 therefore, that the pollen which effected the fertilization of the 

 flowers as shown by the later development of fruit, must have come 

 almost entirely from blossoms Avhich were open when the band 

 pollinating was done. With some of the varieties which were arti- 

 ficially cross-pollinated after the manner above described, a con- 

 siderable portion of the flowers were not opened when the hand 

 pollination was done, and probably did not open for a period of 

 from 24 to 48 hours thereafter. Had the bags been reopened on 

 each of the two days following the one upon which the hand pol- 

 linating was done, and the clusters brushed again with freshly 

 opened blossoms, the result might have given a more accurate in- 

 dication of the kind of clusters which such varieties may be ex- 

 pected to produce when they stand adjacent to each other, and the 

 process of cross-pollination goes on freely during the entire bloom- 

 ing season. 



On the other hand it should be observed in this connection that, 

 even with varieties which had not come into full bloom when the 

 hand pollinating was done, some perfectly filled clusters were 

 obtained, showing that the percentage of blossoms which became 



