32 



Bulletin 125. 



The various cultures and innoculations detailed in the preceding paragraphs 

 were made in the hope that it would be possible, finally, to trace in artificial 

 cultures the exact relation and connection existing between the Tubcradaria, 

 and theperithecial forms of Ncdria and Plconectria. It may be said, how- 

 ever, that at the time of the present writing, after a lapse in some cases 

 of many months, nothing but the stromata of Tubercularia has appeared in 

 the cultures. In exjjlanation one might cite Mayr's observations on the 

 maple (see p. 27). In this case the perithecia did not appear until a full 

 year after the stromata had been formed. It is known, too, that many forms 

 require a much longer time than this to attain to their complete development 

 under artificial conditions. The methods of culture may not have been 



favorable to the pro- 

 duction of the perfect 

 form. It is known 

 tliat many species re- 

 quire a period of rest 

 before such forms will 

 be developed. That a 

 connection exists be- 

 tween Tubercularia 

 vulgaris and Nedria 

 cinnabarina has been 

 abundantly demon- 

 strated, so that the fact 

 is no longer open to 

 question (Mayr, 1. c.) 



Nedria cinnabarina. 



All of the perithecia 

 developed during the 

 fall of 1895, on the 

 currant bushes planted 

 in Fall Creek. Ravine, 

 were those of Pleon- 

 edria, which is not connected with Tubercularia as is shown in a succeed- 

 ing paragraph. 



The canes were examined at intervals during the fall and winter of 

 1895-96, but it was not until Februar}' that a branch was noted, on which 

 clusters were present, which differed decidedly in appearance from those 

 previously observed on this material (Fig. 3, b.) The perithecia were a 

 light brick-red in color, were small, spherical, and densely crowded, and 

 covered with little granules, giving them a rough appearance. There was 

 no depression at the apex as in Pleonedria. The clusters were seated on 

 prominent stromata, evidentl)' those of Tubercularia (Fig. 3, b and 9. ) The 

 tubercles and perithecial clusters were intermingled freely. Many tubercles 



9. Longitudinal section of a stroma and single 

 perthecium 0/ Nedria. 



