34 



Bulletin 125. 



Hum. After three days, the protaplasm became full of vacuoles placed at 

 regular intervals. At this time conidia began to be thrown off from short 

 lateral branches in a manner similar to that previously described. They 

 were 4 — 5 // long, or about the size of those of Tubcrcularia (Fig. ii). 



When the cultures were four days old small portions of the agar contain- 

 ing colonies were transferred to sterilized currant and bean stems. The 

 mycelium spread from the point of innoculatiou until a sparse cottony growth 

 appeared over the stems, especially at the cut ends. Development was more 

 vigorous on the currant than on the bean stems. After nine days growth 

 minute white cushions began to be formed on the surface of the stems. These 

 increased in size, and resembled in every way those previously described for 

 Tubcrcularia. No perithecia have yet been produced in artificial cultures. 



Cultures were also made of the conidia produced on stromata from which 

 perithecia were borne. The germination, mycelial growth, and white cush- 



1 1 . Mycelium of Nectria producing secondary con idia. 



ions produced resembled exactly the like phenomena in the case of TubeV' 



cularia. 



Pleonectria berolinensis. 



Reference has already been made on a previous page to the perithecia 

 of Pleonectria beroline^isis, Sacc. found on one of the currant canes 

 brought from Chautauqua county. This species has long been known as an 

 inhabitant of dead currant stems both in Europe and America. In North 

 America it is said to occur on dead stems of Ribes, in Canada and the North- 

 em United States, west to Montana. 



No reference to this fungus as a parasite has been found. At the time the 

 specimen was collected it was thought that perhaps the species might be 

 another perfect form connected with the Tubcrcularia. The evidence, 

 however, is against this view. 



The currant bushes which had been placed in the ravine, as described on 

 page 26, were left undisturbed during the summer and fall of 1895. About 

 September first, perithecia began to appear abundantly near the bases of the 



