Invasion of the Udder by Bacteria. 



289 



A considerable number of tubes of slanted agar were inoculated 

 with bits of tissue, the size of a pea, taken from the several regions 

 of the six udders comprising group 1, The results are summarized 

 in the following table. 



TABLE No. lY. 

 Agar Slant Cultures Made from TTdders, Group 1. 



Udder 

 No. 



No. 

 inocu- 

 lated. 



15 



8 

 18 

 23 



Source. 



26 Regions A, B and C ol 

 four quarters. 



Milk, right fore quarter. 



Region A, right fore quar 

 ter. 



Region B, right fore quar 

 ter. 



Region C, right fore quar- 

 ter. 



Region C, right fore quar 

 ter. 



5 each from A, B, C, right 

 fore quarter. 



A, B and C of right fore 



quarter. 

 A, B and C of right fore 



quarter. 

 A, B and C of four quar- 

 ters. 



Resulting growth. 



In 23, a confused mass of white, ocher 

 and lemon yellow colored colonies 

 of micrococci. Nos, 1, 2 and 3. 

 In 4, B. subtilis{t) only. 



Pure culture of micrococcus No. 1. 



Pure culture of micrococcus No. 2. 



Micrococcus No. 1 and spore bearer. 



Clear. 



Pure culture of mocrococcus No. 1. 



3 remained sterile, 12 exhibited a con 



fused mass of colonies of species 



1, 3 and 3. 

 Confused mass of colonies of species 



1, 2 and 3. 

 1 from A remained sterile. Remainder 



showed species 1, 2 and 3. 

 6 overgrown by a member of B. sub- 



tilis group. Remainder presented 



a confused mass of species 1, 2 



and 3. 



19 



