Xew Yokk Agkicultukal Expkkiment Stations. 



:]23 



50 cc. of acid to precipitate the casein completely; the exact amount 

 is 44.5 cc. of STF HCl. The remaining amount of base, equal to 

 5.5 cc. of § hydroxide, or 1.1 cc. of ^ hydroxide, appears to be 

 held in combination in the insoluble compound. 



Table VII. — Caseinates of Calcium, Strontium and Barium. 



These results indicate the formation of two sets of compounds, 

 when casein is dissolved in a hydroxide of calcium, strontium or 

 barium and this solution is neutraUzed with acid under the conditions 

 of our experiments. One set of compounds contains twice as much 

 base as the other. 



Attention is called to additional details in the following statements: 



(1) In the di-basic compounds, as the results show, 1 gram of 

 casein requires between 2.2 x 10'* and 2.3 x 10** gram equivalents 

 of hydroxide of calcium, strontium or barium to form a compound 

 which is soluble in water when there is not present any, or more 

 than a trace of, soluble chloride of any of these elements. The 

 addition of even a small amount of a soluble salt of any of these 

 elements to a solution of any of these di-basic caseinates causes the 

 formation of a precipitate. 



(2) In these di-basic compounds, 100 grams of casein combine 



(a) with 0.44 to 0.46 gram Ca (equal to 0.62 to 0.64 gram CaO), 



(b) with 0.96 to 1.01 gram Sr (equal to 1.14 to 1.19 grams SrO), 

 or (c) with 1 . 51 to 1 . 58 grams Ba (equal to 1 . 69 to 1 . 76 grams BaO). 



(3) Apparently, with the treatment described above, 1 gram of 

 casein combines with about 1 . 1 x 10'* gram equivalents of the hydrox- 

 ide of calcium, strontium or barium to form an insoluble compound, 

 when an acid is added in amount just sufficient to precipitate the 

 casein completely. These compounds are regarded as mono-basic. 



(4) In these insoluble mono-basic compounds 100 grams of casein 

 combine approximately (a) with 0.22 gram Ca (equal to 0.31 gram 

 CaO), (b) with 0.48 gram Sr (equal to 0.57 gram SrO), or (c) with 

 . 76 gram Ba (equal to . 85 gram BaO) . 



(5) These insoluble compounds possess some highly interesting 

 properties; they are soluble in a 5 per ct. solution of sodium, potas- 



