00 Report of Department of Animal Industry of the 



FOURTH EXPERIMENT. 



PLAN. 



The cow used in experiment II proved to be a hardy animal, a 

 good milker, and hearty eater, by far the most suitable animal in 

 the herd, and was therefore chosen as the subject of this experiment. 

 When the work began she weighed 495 Ko. and gave nine and one- 

 half kilos of milk. She did not come in regularly, but aborted on 

 February 7. By April 11 she had been adjusted to the follow- 

 ing ration: oat straw 4,536 grams, rice meal 2,724 grams, wheat 

 bran 4,536 grams, wheat gluten 597 grams, which gives a total of 

 249.6 grams nitrogen, and 66.5 grams of phosphorus, 51.1 grams 

 of the latter being in the form of piiytin phosphorus. She was then 

 removed from the herd and placed in a comfortable room planned 

 for metabolism work, and after eighteen days on the ration as given 

 above, samples for analj^sis were taken daily. The days of the 

 experiment were numbered consecutively from the first day of 

 sampling, April 29, to the end of the experiment, eighty-five days 

 later. In the laboratory the samples were known by these numbers. 

 The time was divided into five main periods with transition periods 

 between each two as follows: 

 Period I, days 1-6 (April 29-May 4), The whole wheat bran 



period, with the ration as specified above; 

 Transition period, days 7-10, in which the whole bran was gradually 



replaced by washed bran; 

 Period II, days 11-19 (May 9-May 17), The phosphorus equilib- 

 rium period, in which the intake and outgo of phosphorus were 

 approximately the same, 24.2 grams per day. 

 Period III, days 23-33 (May 21-May 31), The low phosphorus 



period in which^the phosphorus was reduced to fifteen grams; 

 Transition period, on the thirty-fourth day, 50 grams of calcium 

 phytate were added to this ration, this salt was increased to 

 125 grams on the next day; 

 Period IV, days 36-45 (June 3-June 12), The calcium phytate 

 period, which was the same as the preceding period (III) plus 

 one hundred and seventy-five grams of calcium phytate; 

 Transition period, days 46-50, in which decreasing amounts of the 



phytate were added to the ration; 

 Period V, days 51-78 (June 18-July 15), The protracted low- 

 phosphorus period in which the ration was the same as that 

 offered in Period III. 

 The plan included a sixth period identical with the first, in which 

 striking results were anticipated as consequence of the change 

 from the long continued low-phosphorus ration to one high in this 

 element, but pathological conditions developed and the animal was 

 perforce discharged on the eighty-sixth day. 



