134 Kepokt of Department of Animal Industry of the 



The dried substance was analyzed. 

 0.2639 gm. substance gave 0.0452 gm. H2O and 0.0936 gm. CO2. 

 0.1480 gm. " « 0.0866 gm.BaS04 " 0.0846 gm. MgaPsOy. 



0.1632 gm. « « 0.0959 gm. BaS04 " 0.0933 gm. MggPaOy. 



Found C 9.67 per ct., H 1.91 per ct., P 15.93 per ct., Ba 34.43 per ct. 

 H2O 8.33 per ct., P 15.93 per ct., Ba 34.58 per ct. 



A third lot prepared by heating 1.80 gm. dry inosite (1 mol.) with 

 5.88 gm. dry phosphoric acid (6 mol.) and isolating in the same 

 manner as before gave the following: 



C 9.69 per ct., H 1.75 per ct., P 16.06 per ct., Ba 36.33 per ct. 



It is apparent therefore that in each of the above experiments the 

 same compound was produced. 



THE FREE TETRA-PHOSPHORIC ESTER. 



About 5 gm. of the purified barium salt was decomposed by 

 digesting it with the calculated quantity of deci-normal sulphuric 

 acid. After removing the barium sulphate the solution was precipi- 

 tated with excess of copper acetate. The copper precipitate was 

 filtered, thoroughly washed with water, suspended in water and 

 decomposed with hydrogen sulphide. The copper sulphide was 

 removed by filtration, the filtrate concentrated in vacuum and 

 finally dried in vacuum over sulphuric acid until it was of a thick, 

 syrupy consistency. 



For analysis the substance was dried at 130° C. 

 0.3020 gm. substance gave 0.0933 gm. H2O and 0.1577 gm. CO2. 

 0.1605 gm. « " 0.1387 gm. MgaPzOT. 



For C6H6(OH)204 [PO(OH)2]4 = 500. 



Calculated C 14.40 per ct., H 3.20 per ct., P 24.80 per ct. 

 Found C 14.24 per ct., H 3.45 per ct., P 24.09 per ct. 



0.1663 gm. substance ' required 16.5 cc. deci-normal sodium 

 hydroxide using phenolphthalein as indicator. This corresponds to 

 5 acid (OH) groups. 



Calculated for 5 NaOH 16.63 cc. 



properties of the free ester. 



The concentrated aqueous solution of the ester is very similar to 

 phytic acid. It is a very thick, fight, amber-colored liquid of sharp 

 acid, sfightly astringent taste and strong acid reaction. On longer 

 keeping in the desicator over sulphuric acid it becomes hard and 

 brittle and may be powdered. It is then very hygroscopic. 



The dry substance is slowly but completely soluble in alcohol, 

 readily soluble in water. 



