136 Report of Department of Animal Industry of the 



about two hours, until water ceased coming over. The reaction 

 mixture was in form of a very bulky, thin flaky mass, very brittle 

 and of yellowish-brown color, mixed with some very dark-colored 

 substance. It was broken up with a glass rod and removed from 

 the flask and treated with water, in which the dark-colored portion 

 was readily soluble, but the lighter-colored substance was insoluble 

 in this medium. It was powdered in a mortar and thoroughly 

 washed in water and alcohol and dried in vacuum over sulphuric 

 acid. 



The substance was apparently insoluble in boihng water, in boiling 

 dilute acids and in glacial acetic acid; also insoluble in alcohol, ether 

 and other organic solvents. After drying at 130° the substance was 

 analyzed. 



0.2500 gm. substance gave 0.0838 gm. H2O and 0.2085 gm. CO2. 

 0.1500 gm. " " 0.1145 gm.Mg2P207. 



0.1542 gm. « " 0.1178 gm. Mg2P207. 



Found C 22.74 per ct., H 3.75 per ct., P 21.28 per ct., 21.29 per ct. 



This agrees approximately with a mono-pyro-phosphoric ester of 

 inosite, but the phosphorus is too high. 



It was decided to purify it by means of the barium salt. The 

 substance was dissolved by boiling in dilute sodium hydroxide, in 

 which it gave a dark amber-colored solution. After filtering it was 

 precipitated with barium chloride; the barium precipitate filtered 

 and washed free of alkali. It was then dissolved in 500 cc. 0.5 

 per ct. hydrochloric acid and precipitated by barium hydroxide. 

 After filtering and washing it was repeatedly precipitated with 

 alcohol from 0.5 per ct. hydrochloric-acid solution until finally a 

 small amount of a white amorphous powder was obtained. After 

 drying at 130° this was analyzed. 



0.2028 gm. substance gave 0.0412 gm. H2O and 0.0979 gm. CO2. 

 0.2207 gm. " " 0.0413 gm.HzO " 0.1042 gm. CO2. 



0.1982 gm. « " 0.0996 gm. BaS04 " 0.1103 gm. Mg2P207. 



Found C 13.16 per ct., H 2.27^per ct., P 15.51 per ct., Ba 29.57 per ct. 

 C 12.88 per ct., H 2.09 per ct. 



In this compound the relation between the carbon and phosphorus 

 is nearly 6 C to 3 P, which would indicate a tri-phosphoric ester. 

 The substance was, however, far from pure, and lack of material 

 prevented any further investigation of this body, which is apparently 

 a mixture of various esters. 



