146 Report of Department of Animal Industry of the 



cipitated by adding 1 liter of alcohol. The resulting precipitate was 

 filtered off at once and for the purpose of removing adhering inorganic 

 phosphate was precipitated twice from 0.5 per ct. hydrochloric acid, 

 in the presence of a small quantity of barium chloride, with alcohol 

 and then twice from the same strength hydrochloric acid with alcohol. 

 After finally filtering and thoroughly washing in 50 per ct alcohol, 

 alcohol and ether it was dried in vacuum over sulphuric acid. The 

 product so obtained was a white amorphous powder. In appear- 

 ance it was very similar to the tribarium phytate and the barium 

 salt of the tetra phosphoric acid ester of inosite except that when 

 precipitated with alcohol the particles appeared coarser On moist 

 litmus paper it showed a strong acid reaction. It was readily soluble 

 in dilute hydrochloric and nitric acids, less soluble in acetic acid, 

 very slightly soluble in water and exceedingly soluble in 10 per ct. 

 phytic acid. It was free from chlorine. Yield 11 8 gm. 



After drying at 105° in vacuum over phosphorus pentoxide the 

 substance was analyzed. 



0.2617 gm. subst. gave 0.0421 gm. H2O and 0.1016 gm. COo 

 0.2796 gm. subst. gave 0.0488 gm. H2O and 0.1080 gm. CO2 

 0.2566 gm. subst. gave 0.1495 gm. BaS04 and 0.1443 gm. 



MgaPaOy 

 Found: C 10.58: H 1.80: P 15.67: Ba 34.28 per ct. 

 C 10.53: H 1.95 per ct. 



The substance was not yet pure being probably mixed with some 

 monopyrophosphoric acid ester of inosite; at least the high carbon 

 and low phosphorus points to such a conclusion. 



It was hoped that the exceeding solubility of the substance in 

 phytic acid might serve to separate these bodies. For this purpose 

 the whole substance was dissolved in 20 cc. 10 per ct. phytic acid. 

 On diluting with water a portion of the substance separated as a 

 heavy granular powder. To complete the separation 100 cc. of 

 water was added and then allowed to stand two days at room tem- 

 perature. This precipitate was discarded; as analysis, after purifying 

 by precipitating from 0.5 per ct. hydrochloric acid with alcohol, 

 showed that it was still impure and only 0.9 gm. had been obtained. 

 The great bulk of the substance was accordingly contained in the 

 filtrate from the above. This filtrate was diluted to 300 cc. with 

 water and then precipitated by adding 300 cc. alcohol. The volu- 

 minous white precipitate was filtered off, washed thoroughly in 50 

 per ct, alcohol and alcohol. For purification it was dissolved in 

 0.5 per ct. hydrochloric acid and precipitated by alcohol. After 

 filtering and thoroughly washing in dilute alcohol until free from 

 chlorine it was washed in alcohol and ether and dried in vacuum 

 over sulphuric acid. The product so obtained was a pure white 

 amorphous powder. On moist litmus paper it showed a strong 



