150 Report of Department of Animal Industry of the 



preparation of the free di-inosite tri-pyrophosphoric acid 



ester. 



The purified dry barium salt (1.5 gm.) was suspended in water, 

 decomposed with shght excess of sulphuric acid, the barium sulphate 

 removed and the solution precipitated with copper acetate. The 

 copper salt was decomposed and the free ester obtained in exactly 

 the same way as described for the di-pyrophosphoric ester. 



In this case also the copper sulphide could be precipitated only 

 after the solution had been acidified with hydrochloric acid. For 

 the removal of the hydrochloric acid the filtrate was evaporated 

 several times in vacuum with water and finally dried in vacuum over 

 sulphuric acid and potassium hydroxide. The product, like the 

 previous compound, was a thick, light amber colored syrup. For 

 analysis it was dried at 105° C. 



0.1607 gm. subst. gave 0.0466 gm. H2O and 0.0744 gm. CO,. 

 0.1083 gm. subst. gave 0.1030 gm. Mg2P207. 



For Ci2H3204iPio= 1142 



Calculated C 12.60: H 2.80: P 27.14 per ct. 



Found C 12 62: H 3.24: P 26.51 per ct. 



PROPERTIES OF THE DI-INOSITE TRI-PYROPHOSPHORIC ESTER 



The properties of this ester agree in the main with those mentioned 

 of the di-pyrophosphoric ester. The concentrated solution of the 

 ester forms a thick, light amber colored syrup which on longer drying 

 in desiccator becomes brittle and hygroscopic. The aqueous solution 

 is of strong acid reaction and pleasant acid taste. The precipitates 

 produced with calcium, magnesium, silver and iron salts are identical 

 with those given by the di-pyrophosphoric ester. 



Barium chloride produces at once a white precipitate sparingly 

 soluble in acetic but readily soluble in dilute hydrochloric and nitric 

 acids. 



Ordinary molybdate solution produces a white precipitate which 

 does not turn yellowish in color, being in this respect identical with 

 phytic acid. 



Neutral molybdate solution causes at first a voluminous white 

 precipitate which redissolves almost immediately. The addition of 

 a few drops of the ordinary acid molybdate to this solution and 

 scratching with a glass rod causes the separation of long white needle- 

 shaped crystals. The crystals and the precipitate caused by the 

 ordinary molybdate solution are readily soluble in ammonia. 



On drying at 105° the substance turns very dark in color. 



Lack of material prevented the hydrolysis of this ester and the 

 isolation of inosite as one of the products of decomposition had 

 therefore to be omitted. 



