New York Agricultural Experiment Station. 157 



substance from wheat bran and hope shortly to be able to report 

 further concerning this compound. Various other cereals and 

 feeding stuffs are also being examined to determine if they contain 

 phytin or if this other organic-phosphoric acid compound is 

 present. 



EXPERIMENTAL. 



preparation of the organic-phosphorus compound from 



WHEAT bran. 



The bran was digested with 0.2 per ct. hydrochloric acid over 

 night and the extract after straining and filtering was precipitated 

 with alcohol according to the method of Patten & Hart (loc. cit.). 

 The resulting precipitate was purified by precipitating five times 

 from 0.2 per ct. hydrochloric acid with alcohol. From 500 grams 

 bran 2.5 grams substance was obtained as a white amorphous non- 

 hygroscopic powder. It is readily soluble in its own weight of 

 water, forming a thick light-amber-colored solution of pleasant 

 and characteristic but faint acid odor. The substance reduces 

 Fehling's solution on boiling and it gives the orcine and phloroglucine 

 reactions. The aqueous solution is of acid reaction on litmus 

 paper. It is precipitated by alkahes and solutions of salts of other 

 metals. Warmed with dilute nitric acid and ammonium molybdate 

 it does not give any immediate precipitate but on standing for 

 several hours a trace of yellow phosphomolybdate is precipitated 



After drying at 120° the substance was analyzed 



0.1642 gram substance gave 0.0522 gm. H2O and 0.1285 gm. CO2. 



0.0860 gram substance gave 0.0428 gm. Mg2P207 for P. 



0.1720 gram substance gave 0.0264 gm. Mg2P207 for Mg. 



0.1720 gram substance gave 0.0054 gm. CaO. 



0.3897 gram substance gave 0.0085 gm. N (Kjeldahl). 



The substance contained only a very small quantity of K. 



Found C 21.34, H 3.55, P 13.87, Ca 2.24, Mg 3.35, N 2.18 per ct. 



A larger quantity of the product was then prepared by extracting 

 3 kg. of bran. After isolating and purifying in the same way as 

 before 47 grams substance was obtained or about 1.5 per ct. of the 

 weight of the bran used. In appearance and properties it was 

 identical with the foregoing. 



After drying at 105° in vacuum over phosphorus pentoxide tlie 

 substance was analyzed. 



0.2444 gram substance gave 0.0710 gm. H2O and 0.1940 gm. CO2. 



0.3936 gram substance gave 0.0149 gm. CaO and 0.0861 Mg2P207. 



0.3936 gram substance gave 0.0623 gm. K2 PtCle. 



Phosphorus and nitrogen determinations were not made. 



Found C 21.64, H 3.25, Ca 2.70, Mg4.77, K 2.54 per ct. 



