158 Report of Department of Animal Industry of the 



The substance was again precipitated from 0.2 per ct. hydrochloric 

 acid, washed and dried as before when the following results were 

 obtained on analysis: 



0.1934 gram substance gave 0.0639 gm. H2O and 0.1523 gm. CO2. 



0.3313 gram substance gave 0.0131 gm. CaO and 0.0717 gm. 

 Mg2P207 for Mg. 



0.3313 gram substance gave 0.0444 gm. K2 PtCle. 



0.1656 gram substance gave 0.0857 gm. Mg2P207 for P. 



0.4639 gram substance gave 0.0058 gm. N (Kjeldahl). 



Found C 21.47, H 3.69, P 14.42, Ca 2.82, Mg 4.72, K 2.15, 

 N 1.25 per ct. 



Sodium was not determined but qualitative tests showed that it 

 was present. 



The reprecipitated substance, 0.3887 gm., distilled with 12 per ct. 

 HCl gave 0^0367 gm. phloroglucid. 



As the composition did not change by reprecipitation it was 

 deemed sufficiently pure to use in the subsequent experiments* 



It was thought at first that this substance might be phytin mixed 

 with some carbohydrate and some basic nitrogen body. In the 

 hope of separating these and to obtain pure compounds the sub- 

 stance was treated with barium hydroxide and the resulting barium 

 salt purified as follows: 



PREPARATION OF THE BARIUM SALT. 



Five grams of the substance were dissolved in 10 cc. of water and 

 the solution diluted to 200 cc. with water. Barium hydroxide was 

 then added until distinctly alkaline and the mixture heated nearly 

 to boiling. It was then filtered hot and washed with hot water, 

 the filtrate being reserved for examination. 



The washed barium precipitate was dissolved in just sufficient 

 0.5 per ct. hydrochloric acid, filtered, again precipitated with barium 

 hydroxide, the resulting precipitate dissolved by the careful addition 

 of dilute hydrochloric acid and then precipitated by the addition 

 of a like volume of alcohol. The substance was filtered, washed 

 in dilute alcohol, again dissolved in 0.5 per ct. hydrochloric acid 

 and precipitated in the same manner as before. These operations 

 were repeated four times. It was then dissolved in the same strength 

 hydrochloric acid, precipitated with alcohol, filtered, washed in 

 dilute alcohol, alcohol and ether and dried in vacuum over sulphuric 

 acid, The product was a perfectly white amorphous powder. 

 Yield 3.9 grams. 



The substance was slightly soluble in boiling water. On cooling, 

 however, it does not crystallize out and on concentrating in vacuum 

 it separates in an amorphous form. Alcohol also produces a white 

 amorphous precipitate. Various other methods were tried to obtain 

 the substance in crystalline form but without success. On moist 



