New York Agricultural Experiment Station. 159 



litmus paper it shows a strong acid reaction. It was free from 

 nitrogen. 



As it was found impossible to crystallize the substance it was 

 analyzed directly after drying at 130°. 



0.2564 grams substance gave 0.0604 gm. H2O and 0.1314 gm. CO2. 



0.2903 grams substance gave 0.1544 gm. BaS04 and 0.1324 gm. 



Mg;:P207. 



Found C 13.97, H 2.63, P 12.71, Ba 31.29 per ct. 



Of this substance 1.2124 gm. was distilled with 12 per ct. HCl 

 when 0.0053 gm. phloroglucid was obtained. 



The composition of the above salt is entirely different from that 

 of a barium phytate. The relation of the numbers found lead to 

 the empirical formula C25 H55 O54 P9 Ba5=2185. 



Calculated for this C 13.73, H 2.51, P 12.76, Ba 31.44 per ct. 



examination of the filtrate from thh above compound after 

 precipitating with barium hydroxide. 



The filtrate was of light amber color. The excess of barium 

 hydroxide was removed with carbon dioxide, filtered and the filtrate 

 concentrated on the waterbath; again filtered from traces of barium 

 carbonate and then dried in vacuum over sulphuric acid. There 

 remained a small quantity of a yellowish amber-colored, somewhat 

 gummy mass. It contained a large quantity of nitrogen. It did 

 not reduce Fehling's solution and gave only a faint biuret reaction. 



The substance is readily soluble in water and is again precipitated 

 by alcohol but it is not precipitated by tannic acid. The aqueous 

 solution acidified with nitric acid gives no reaction with ammonium 

 molybdate. 



After combustion the ash was found to contain potassium, sodium 

 and phosphorus. When the crude substance is treated by the 

 Van Slyke method for amino nitrogen a small quantity of nitrogen 

 is liberated. 



Lack of time has prevented the further examination of this body 

 and it has not been isolated in pure form. 



ISOLATION OF THE FREE ACID FROM THE FOREGOING BARIUM SALT. 



The barium salt (3.2 grams dry substance) was suspended in 

 100 cc. of water and decomposed with a slight excess of dilute sul- 

 phuric acid; the barium sulphate was removed by filtration and 

 the filtrate precipitated with excess of copper acetate. The copper 

 salt was filtered, thoroughly washed in water, suspended in water 

 and decomposed with hydrogen sulphide. The copper sulphide 

 was filtered off and the filtrate concentrated in vacuum to small 

 bulk and finally dried in vacuum over sulphuric acid until it was 

 of a thick syrupy consistency. After drying at 100° to constant 

 weight the substance was analyzed. 



