1G2 Report of Department of Animal Industry of the 



ture in a well-closed Erlenmeyer flask the substance separated 

 slowly in long white silky needle-shaped crystals. 



In the absence of chloroform or in more concentrated solutions 

 only amorphous white precipitates are obtained. 



The crystals were filtered off and washed in a mixture containing 

 equal parts of alcohol and ether and finally in ether and dried in 

 the air. Yield about 0.5 grams. 



The substance is very soluble in water, readily soluble in alcohol, 

 but insoluble in ether or chloroform. 



Heated in a capillary tube the substance melts at 196°-198°, but 

 the melting point is not sharp. On moist litmus paper it shows a 

 strong acid reaction. It loses weight on drying corresponding to 

 30 H2O. The dried substance was analyzed. 



0.1364 gm. substance lost 0.0124 gm. H2O and 0.1308 gm. sub- 

 stance lost 0.0118 gm. H2O. 



0.1240 gm. substance gave 0.0694 gm. H2O and 0.2557 gm. CO2. 



0.1383 gm. substance gave 0.0233 gm. Mg2P207. 



0.1190 gm. substance gave 6.1 cc. nitrogen by 16° and 746 mm. 



For C20 H55 O49 P9 (C23 H26 O4 N2) 10 = 5298. 



Calculated C 56.62, H 5.94, P 5.26, N 5.28 per ct. 



Found C 56.24, H 6.26, P 4.69, N 5.86 per ct. 



Calculated for 30 H2O 9.24 per ct.; found 9.09 and 9.02 per ct. 



ACTION OF DILUTE SULPHURIC ACID ON THE BARIUM SALT. 



Five grams of the air-dried salt, C20 H45 O49 P9 Bas, were boiled 

 for one hour under a reflux condenser with 100 cc. N/1 H2 SO4. The 

 reaction mixture was precipitated with slight excess of barium 

 hydroxide, filterd and washed with water. The filtrate was exam- 

 ined as mentioned below. 



The barium precipitate was shaken up with 300 cc. 0.5 per ct. 

 hydrochloric acid and the insoluble portion filtered off. To the 

 filtrate was added an equal volume of alcohol and the white flocculent 

 precipitate filtered off and washed in dilute alcohol. It was again 

 dissolved in 0.5 per ct. hydrochloric acid, precipitated with alcohol, 

 filtered, washed free of hydrochloric acid with dilute alcohol and 

 then in alcohol and ether and dried in vacuum over sulphuric acid. 

 Yield 2 grams. The product was a white amorphous powder. After 

 drying at 120° the following results were obtained on analysis: 



Found C 11.64, H 2.25, P 13.95, Ba 33.26 per ct. 



This corresponds exactly with the composition of the substance 

 before treatment with N/1 H2 SO4. It is apparent therefore that no 

 partial decomposition takes place. 



EXAMINATION OF THE FILTRATE FROM ABOVE. 



The excess of barium hydroxide was removed with carbon dioxide 

 and, after filtering, the filtrate was concentrated in vacuum at a 



