164 Report of Department of Animal Industry. 



coarser and may then be easily removed by simple filtration. The 

 resulting filtrate is nearly colorless or of fight amber color. Alcohol 

 produces in this solution a nearly colorless precipitate which is much 

 more easily purified than the product obtained without first precipi- 

 tating with tannic acid. 



With only this modification some of the substance was prepared 

 from wheat bran. It was found, however, to differ slightly in com- 

 position from that obtained by the first method. On analysis the 

 following results were obtained: C 19.51, H 3.09, P 15.23, Ca 0.38, 

 Mg 7.35, K 2.75, N 0.57 per ct. 



On treating this substance with barium hydroxide and purifying 

 the resulting precipitate in the same way as before the same barium 

 salt was obtained: 



For C25H55 054P9Ba5 =2184. 



Calculated C 13.73, H 2.51, P 12.76, Ba 31.44 per ct. 



Found C 13.00, H 2.46, P 12.47, Ba 33.00 per ct. 



The difference in composition of the crude substance must there- 

 fore be due to the smaller amount of the nitrogen-containing body 

 which this preparation was found to hold. In the analysis of the 

 crude substance only 0.57 per ct. nitrogen was found, whereas the 

 first preparation had four times, and the second preparation two 

 times as much. 



isolation of the substance as a barium salt directly from 



the bran extract. 



The bran was digested with 0.2 per ct. hydrochloric acid over 

 night The strained extract was precipitated with tannic acid^ 

 filtered, and a solution of barium chloride added, which caused a 

 small precipitate to separate. An equal volume of alcohol was then 

 added. After settling, the precipitate was filtered and purified as 

 follows: The substance was dissolved in 0.5 per ct. hydrochloric 

 acid, precipitated with barium hydroxide in excess, filtered, again 

 dissolved in the same strength hydrochloric acid and then precipi- 

 tated with alcohol. It was then precipitated a second time with 

 barium hydroxide, and after that precipitated from 0.5 per ct. 

 hydrochloric acid with alcohol until the product did not give any 

 reaction with ammonium molybdate. 



A white amorphous powder was finally obtained. On moist 

 litmus paper it showed a strong acid reaction. After drying at 105° 

 in vacuum over phosphorus pentoxide it was analyzed: 



0.2870 gm. substance gave 0.0630 gm. H2O and 0.1584 gm. CO2. 



0.3066 gm. substance gave 0.0653 gm. H2O and 0.1700 gm. CO2. 



0.2632 gm. substance gave 0.1437 gm. BaS04 and 0.1020 gm. 

 MgsPsOv. 



Found I C 15.05, H 2.45, P 10.80, Ba 32.12 per ct. 

 II C 15.12, H 2.38. 



