172 Report X)F Department of Animal Industry of the 



0.2450 gram subst. lost 0.0272 gm. H2O on drying 



0.2178 gram subst. gave 0.0284 gm. H2O and 0.0522 gm. CO2 



0.1776 gram subst. gave 0.1170 gm. BaS04 and 0.1078 gm. Mg2P207. 



Found C = 6.53; H = 1.45; P = 16.91; Ba = 38.76; H2O = 11.10 per ct. 



This substance agrees nearly in composition with tribarium phytate. 



Calculated for tribarium phytate, C6Hi8027P6Ba3 = 1120. 



C = 6.42; H = 1.60; P = 16.60; Ba = 36.78; 8H20 = 11.39 per ct. 



The amorphous product (7.4 grams) mentioned above was analyzed 

 after previous drying at 105° in vacuum over phosphorus pentoxide 

 and the following results obtained : 



C = 8.04; H = 1.62; P = 16.65; Ba = 36.55 per ct. 



The substance was free from chlorine. It was very slightly 

 soluble in boiling water. With phloroglucine it gave a cherry red 

 color; with orcine only a faintly, greenish color was produced. 

 After boiling in dilute hydrochloric acid, precipitating the barium 

 with sulphuric acid, filtering and neutralizing, it reduced Fehling's 

 solution slightly on boiling. Evidently some carbohydrate was still 

 present. 



For further purification the substance was dissolved in 0.5 per ct. 

 hydrochloric acid, filtered and alcohol added until a faint permanent 

 turbidity remained. This was cleared up by the addition of a few 

 drops of dilute hydrochloric acid and the solution allowed to stand 

 at room temperature. The substance soon began to separate in 

 the same crystalline form as before. After standing for two days 

 the crystalline substance was filtered off, washed in water, alcohol 

 and ether and dried in vacuum over sulphuric acid. 



The mother liquor was diluted with more alcohol and allowed to 

 stand as before when a further quantity of the same shaped crystals 

 was obtained. After filtering, washing and drying as before these 

 salts were analyzed after first drying at 105°. 



Found, first crop of crystals: 



C = 7.06; H = 1.53; P = 16.46; Ba = 38.16 per ct. 



Found, second crop of crystals: 



C = 7.47; H = 1.58; P = 16.46; Ba = 38.12 per ct. 



In order to determine if further treatment would alter the com- 

 position the whole substance was digested in 50 per ct. acetic acid 

 over night, filtered, washed in water, alcohol and ether and dried in 

 the desiccator. It was then dissolved in 0.5 per ct. hydrochloric 

 acid, filtered and the solution brought to crystallization by the 

 careful addition of alcohol as before. The product finally obtained 

 weighed 3.8 grams and it was a snow-white crystalline powder. 

 For analysis it was dried at 105° in vacuum over phosphorus pent- 

 oxide. Found: C = 7.10; H = 1.52; P= 17.17; Ba = 38.11 per ct. 

 As continued treatment did not alter the composition and as it 

 separated in crj^stalline form it was undoubtedly a homogeneous 

 compound. 



