174 Report of Department of Animal Industry. 



washings from above were united and precipitated by alcohol. 

 After standing over night the amorphous precipitate had changed 

 to the usual crystalline form. After filtering, washing and drying in 

 vacuum over sulphuric acid it weighed 14.7 grams. 



The above salts were free from chlorine. The nitric acid solutions 

 gave no immediate reaction with ammonium molybdate. No 

 appreciable color reactions were obtained with phloroglucine or 

 orcine and they did not reduce Fehling's solution. Metals other 

 than barium were absent. For analj'sis the substances were dried 

 at 105° in vacuum over phosphorus pentoxide. 



The first crystalline compound gave the following: C = 6.05; 

 H = 1.45; P= 16.51; Ba = 40.04; H20 = 12.06 per ct. This salt is 

 evidently a mixture of the tri- and tetrabarium salt. It was recrys- 

 tallized as follows: One gram of the substance was dissolved in 

 about 150 cc. of 0.5 per ct. hydrochloric acid and the free acid nearly 

 neutralized with barium hydroxide. About 0.5 gram of barium 

 chloride was then added and the solution allowed to stand for two 

 days at room temperature. The substance separated slowly in the 

 same general crystal form as before, except that the individual 

 crystals were much larger. These were filtered, washed in water, 

 alcohol and ether and dried in the air. Under the microscope the 

 substance appeared homogeneous. Yield 0.9 grams. After drying 

 at 105° in vacuum it was analyzed: 



0.4067 grams subst. lost 0.0496 grams on drying. 



0.3571 grams subst. gave 0.0358 grams II2O and 0.0699 grams CO 2. 



0.2068 grams subst. gave 0.1530 grams Ba SO4 and 0.1160 grams 



Mg2P207. 



Found: C = 5.33; H = 1.12; P = 15.63; Ba = 43.53; H20 = 12.19 

 per ct. 



Calculated for tetrabarium phytate C6Hi6027P6Ba4 = 1255. 



C = 5.73; H = 1.27; P- 14.82; Ba = 43.74; 10 HaO^ 12.54 per ct. 



The second crystalline compound mentioned above gave the fol- 

 lowing result on analysis: 



C-6.88; H = 1.50; P= 15.94; Ba = 37.38 per ct. 



P= 16.28; Ba = 37.21 per ct. 



This corresponds to a tribarium salt. 



A further quantity of the free acid was prepared from this salt by 

 the usual method. From 7 grams of the substance practically the 

 theoretical quantity of acid was obtained. After drying at 100° the 

 substance was analyzed: 



0.2319 grams subst. gave 0.0767 grams H2O and 0.0925 grams CO2. 



After drying over boiling chloroform over phosphorus pentoxide: 

 0.2378 grams subst. gave 0.0703 grams H2O and 0.0961 grams CO2. 

 0.1495 grams subst. gave 0.1414 grams Mg2 P2 O7. 



Found C = 10.87; H = 3.70 per ct. 



C= 11.02; H = 3.30; P = 26 . 36 per ct. 



