60 Report of the Department of Bacteriology of the 



most favorable for obtaining a true sample of the milk, that is, after 

 a thorough stirring of the contents of the pail. 



In view of the favorable results obtained by stirring the milk with 

 a sterile spoon a comparison was made between such samples and 

 those obtained by pouring. In this case samples were collected in 

 sterile test tubes as the milk was poured from the milking machine 

 into sterile pails. The milk was then stirred thoroughly with 

 a long-handled sterile spoon and a second sample collected. The 

 technique of plating and incubation was the same as with the pre- 

 ceding samples. 



The results from 23 such sets of samples are given in Table V. 



Table V. — Germ Content of Samples Obtained by Pouring and by Spoon. 



Date. 



1909 

 June 21 



22 



23 



24 



25 



26 



Totals 



Average 



Highest 



Lowest 



Poured Sample. 



No. 



1 

 10 

 4 

 2 

 2 

 1 



16 

 3 

 1 

 6 

 3 

 3 

 1 

 2 



1 



1,780 

 930 

 171 

 724 

 265 

 686 

 637 

 426 

 076 

 766 

 791 

 423 

 850 

 660 

 756 

 373 

 708 

 583 

 008 

 726 

 435 

 623 

 448 



76 

 3 



845 

 341 



Rank. 



+ 

 + 



+ 



+ 



IS 



Spoon Sample. 



No. 



1 

 1 

 1 

 14 

 5 

 2 

 3 

 2 



20 

 4 

 1 



18 

 2 

 3 

 2 



1 



1 

 1 

 1 

 2 

 2 

 2 

 1 



101 



4 



813 

 311 

 351 

 800 

 020 

 788 

 000 

 531 

 626 

 173 

 511 

 533 

 700 

 840 

 096 

 550 

 113 

 913 

 401 

 896 

 538 

 556 

 425 



545 

 310 



Rank. 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



+ 



+ 

 + 



+ 



+ 

 + 

 + 



18 

 5 



From the above results it is seen that the samples taken with a 

 sterile spoon not only gave a distinctly larger average germ count 



