112 Report of the Department ok Bacteriology of the 



representatives were found. The absence of this genus was noted 

 early in the qualitative work and attention given to the detection 

 of streptococci. Neither of the two forms here listed are typical 

 members of the genus, their tendency to form chains being rather 

 weak. It will also be noted that two strains of Streptococcus 

 lactieus are among the named species with an interrogation point. 

 These strains were not streptococci but agreed fairly closely with 

 the description of that species in other respects. Many of the 

 streptococci, which have been isolated from other sources, grow 

 rather poorly upon the standard agar used in this study and it is 

 possible that additional forms would have been found here had 

 special media 1 ' been employed in the search. 



The two non-spore-bearing yeasts were also unexpected forms 

 but were so constantly present in large numbers in at least one 

 udder at> to be undoubted members of the udder flora. No spores 

 were observed in these yeasts even when grown on gypsum blocks,, 



The greater number — 75 per ct. — of these forms were in- 

 cluded in the genus Micrococcus. This is quite in accord with 

 the experience of other students who have found udder cocci 

 especially abundant. 



There is an entire absence of spore formation among the or- 

 ganisms here described as representatives of the udder flora. This 

 is not so unusual as would at first appear since 77 per ct. of these 

 forms are cocci, among which spore formation is unknown. 



Strict anaerobes are lacking in the above list since the technique 



employed would not have shown those which may have been 



present. Facultative anaerobes make up 20 per ct. "of the list and 



the remaining 80 per ct. are classed as strict aerobes. This 



classification of the oxygen relation of the forms was made on 



the basis of their growth in the closed arm of the fermentation 



tube. Either this basis of classification is not accurate or the 



aeration within the udder is vastly different from what one would 



expect from the anatomy of the organ. Tf free oxygen is absent 



17 North, C. E. An agar gelatin medium. Jour, of Med. Research 20:359- 

 3G3. 1909. 



