New York Agricultural Experiment Station. 379 



pollen cultures in mixture o. 



The mixture which for convenience of reference is here called 

 C consisted of a Bordeaux mixture made wholly of chemically 

 pure ingredients combined with a solution of cane sugar in 

 water-v The formula used was 1 gram of copper sulphate and | 

 gram of lime to make 100 cc. Bordeaux mixture.^ After the cop- 

 per sulphate solution and lime had been combined and diluted 

 to 96 cc. with distilled water 4 cc. of 50 per ct. aqueous cane 

 sugar solution were added to bring the whole mixture up to 

 100 cc. Work with mixture C did not begin until after apples 

 were out of bloom and so the results given with it were obtained 

 with other kinds of pollen than that used for the cultures in 

 mixtures A and B. 



Van Tieghem cell cultures which were made in the way 

 previously described, p. 375, were prepared June 28. In each 

 one was put some blackberry pollen from the same anther. The 

 following statement shows the medium used for each culture. 



Seeies III. 



Cxiltnre Nnmber of pollen 



No. Medium. grains in cnltnre. 



15 Mixture C, 100 parts 128 



16 Mixture C, 2 parts; 2 per ct. aqueous solution of 



cane sugar, 98 parts 115 



17 Mixture G, 1 part; 2 per ct. aqueous solution of 



cane sugar, 99 parts 108 



18 Mixture C, ^^^ part; 2 per ct. aqueous solution of 



cane sugar, 99^4 parts 64 



19 Mixture 0, 5!! part; 2 per ct. aqueous solution of 



cane sugar, 99 i?, parts 43 



20 Copper arsenite and lime, 1 gram to 1^ liters; in 



2 per ct. aqueous solution' of cane sugar About 12 



21 Lime, 1 gram to 1.25 liters of a 2 per ct. aqueous 



solution' of cane sugar 69 



22 Cane sugar, 2 per ct. aqueous solution 107 



^This is the same as 10 grams copper sulphate to make 1 liter of Bor- 

 deaux mixture which formula is nearly equivalent to the 1-to-ll formula. 

 See p. 375. 



'This Is at the rate of about 1 lb. to 150 gallons. 



'The copper arsenite, CuHAsOs, was used at the rate of 1 gram to 1.25 

 liters. This is approximately the strength at which either this or Paris 

 green is used in orchard spraying; i. e., about 1 lb. to 150 gallons. Lime 

 was added at the same rate as the copper arsenite: 1. e., 1 gram to 1.25 

 liters, and cane sugar solution at a rate to make the whole a 2 rer ct. 

 solution of cane sugar. 



