SSO Report of the Horticultural Department of the 



When preparation of these cultures was finished, at 5 p. m., 

 June 28, they were put into moist chambers and kept in a room 

 haying nearly constant temperature of about 70° to 72° F. 

 When they were examined at 8.40 a. m., June 29, no pollen had 

 germinated in any of them except in No. 22 which contained 

 2 per ct. sugar solution in water only. ^ In this culture out of 

 107 pollen grains 9, or about 8 per ct., had germinated. 



The cultures were kept four days, till July 2, and then dis- 

 carded. During this time they were kept in the room above* 

 mentioned except that on one day, June 29, they were for about 

 ten hours in a room where the temperature ranged from 80° F. 

 to 82° F. On July 2 the following notes were made; 



Xo. 15 shows no germination. 



No. 16 ditto. 



No. 17 ditto. 



Kg. 18. ditto. 



Ko. 19. Twelve pollen grains, or 28 per ct., show evidence of 

 germination. In these cases the germ tube has only attained 

 a length of from one-half to two-thirds the diameter of the pol- 

 len grain and then disintegration has followed. 



No. 20 shows no germination. 



No. 21 destroyed by accident. 



No. 22 shows 52 germinations or 49 per ct. 



About 11.30 a. m., June 19, the following cultures were made 

 with rose pollen. 



Seeiks IV. 



Cnlttire NnTuber o( pollen 



Xo. Medium, grains iu cnlluie, 



30 ^Mixture C, 1 part; aqueous solution of cane sugar, 



99 parts About 150 



31 Cane sugar, 2 parts; water, 98 parts About 150 



These cultures were kept in moist chambers iu the laboratory 

 till 5.30 p. m., or 6 hours. There were then no germinations in 

 No. 30, but 36 pollen grains out of about 150 in culture No. 31 

 had already germinated. The cultures were then kept in the 



