Kew York Agricultural Experiment Station. 381 



room having a temperature of 70^ F. On July 2, after being 

 kept three days many more germinations were found in No. 21 

 but none in No. 30. 



Cultures of pollen of the Virginia Creeper, Ampelopsis quin- 

 quefolia, all from the same anther, were made at 11:30 a, m., 

 July 25, 1900, as listed below. 



Series V. 



NnmLer Per ct. 



of pollen Nnniljer ofger- 



Culture grains in gertni- niina- 



>.o. Mediuin. culture. nated. tion. 



o'j 2 per ct aqneous cane sugar solution, 



2 parts; water, 98 parts 88 66 75 



30 Lime, 1 gram to 125 cc. of a 2 per ct. 



aqueous solution of cane sugar 144 



ST Copper arsenite and lime^ and a 2 per 



ct. aqueous solution cane sugar 2.30 



38 Mixture C, bV part; aqueous solution of 



cane sugar 99JS parts 203 136 67 



39 Mixture C, I/2 part; aqueous solution of 



cane sugar, 99',^ parts 118 5 4 



40 Mixture C, 1 part; aqueous solution of 



cane sugar, 1 part 187 



41 Mixture C, 2 parts; aqueous solution of 



cane sugar, 98 parts 122 



After six hours it was found that many pollen grains had ger- 

 minated in Nos. 35 and 38. Five had also germinated in No. 39. 

 There were no germinations in any of the other cultures. At 

 8:30 a. m., July 26, after the cultures had been made 21 hours 

 it was found that there were 66 germinations in No. 35, or 75 

 per ct; in No. 38 there were 130 or 67 per ct.; and in No. 39 there 

 were 5, or 4 per ct. In none of the other cultures was any ger- 

 mination found. After being kept 24 hours longer no further 

 germination was found and these cultures were discarded. 



Cultures of the pollen of Japan ivy, or Boston ivy, Ampelopsis 

 tricuspidata, were made at 11:30 a. m., July 30, using the same 

 media as in Nos. 37 to 41, and also one culture was made in a 

 2 per ct. sugar solution in water alone. 



^Prepared as for culture No. 20, seo p. 379. 



