12 A SPOT DISEASE OF CAULIFLOWEE. 



pale green-fluorescent (between water green and greenish glaucous, 

 Repertoire de Couleurs, Paris, 1905); more precipitate than in beef 

 bouillon. 



Nutrient gelatin (+10 on Fuller's scale). — The stab cultures lique- 

 fied in 8 to 10 days (temperature 17° to 18° C). Growth from sur- 

 face crateriform. Slight, white, granular precipitate. Slight green 

 fluorescence. 



The plate cultures showed no signs of growth in 24 hours at 17° to 

 18° C. In three days well-isolated colonies vary from mere points to 

 round growths 2 millimeters in diameter. The gelatin is liquefied in 

 cuplike hollows. Margin of smaller colonies entire, of larger colonies 

 fimbriate. Tliicklv sown plates entirely liquefied in two days at 15° 

 to 16° C. ^ -^ 



Litmus milk. — TJie medium becomes dark blue at the surface in 

 12 to 24 hours. The darkening proceeds downward in definite layers 

 until in 8 to 10 days the whole medium is dark blue with a slight white 

 precipitate. During six months' observation the medium remained 

 dark blue (reflected light) and liquid. Finalh^^ by evaporation the 

 medium becomes thickened, but there is at no time any separation 

 into curd and whey. 



A few cultures showed a trace of reduction of litmus at the bottom. 



Milk. — As in the litmus-milk cultures, growth and color-change in 

 the milk begin at the surface, proceeding downward in definite layers. 

 In 15 to 20 days the whole tube (10 c. c. of milk) is yellow (near 

 Ridgway's Naples yellow, but somewhat duller and with a greenish 

 tinge) and translucent. No separation into curd and whey. Fat 

 not changed. In four months the medium is quite dark (reddish- 

 brown) and somewhat thick (evaporated to about 5 c. c). Small 

 tyrosin crystals are formed. These are distinctly visible only with 

 a lens. 



UschinsTcy's solution. — Growth moderate to copious; pellicle white, 

 tender, breaking and sinking easily. Pseudozoogloese are present. 

 There is a greenish fluorescence. The old cultures are much like 

 those in Fermi's solution. 



OTHER CHARACTERISTICS. 



Fermentation tubes. — The organism is aerobic and does not form gas. 

 It was tested in fermentation tubes in the presence of dextrose, 

 saccharose, lactose, maltose, gl3^cerin, and mannit, each of these 

 carbon compounds being added to a basal solution consisting of 1 

 per cent of Witte's peptone dissolved in water. It did not grow in 

 the closed end of the fermentation tubes in the presence of any of 

 these substances. 



Ammonia 'production. — Moderate. 



225 



