6 HISTOEY AND CAUSE OF THE COCONUT BUD-EOT. 



Laboratory and greenhouse studies of the disease — Continued . 



Cultural experiments — Continued. Vage. 



Special test reactions for the identification of the organism 77 



Dolt's synthetic medium No. 1 79 



Neutral red used in various media 80 



MacConkey's bile-salt agar with neutral red 83 



Test 1 of D . Rivas 85 



Test 3 of D. Rivas 85 



Growth on Endo's fuchsin agar 88 



Stoddart's plate medium 91 



Hiss's tube medium 91 



Growth in sterile milk 92 



Growth in litmus milk 92 



Products of growth of the organism 92 



Production of indol and phenol 92 



Production of hydrogen sulphid 93 



Production of ammonia 93 



Enzymes in milk 94 



Production of alcohols, aldehydes, and acetone 96 



Production of volatile and fixed acids 97 



Reduction of colors 100 



Growth on miscellaneous culture media 101 



Nitrogen-free media 101 



Fischer's mineral solution with various nutrient substances 104 



Media with malachite green 106 



Beef agar containing caffein 107 



The media of Capaldi and Proskauer 107 



Beef bouillon of various degrees of acidity 109 



Dunham's solution with various proportions of sodium chlorid 111 



Uschinsky's solution 113 



Cohn's solution 114 



Potato agar 114 



Carrot agar 114 



Litmus-lactose agar 114 



Oxalic-acid agar 114 



Mercuric chlorid 114 



Monocalcium phosphate 115 



Peptone solution containing rosolic acid 115 



Albumin 116 



Succinic acid 116 



Coconut cylinders 117 



Test 2 of D . Rivas 118 



Peptone with levulose, galactose, and mannit in fermentation 



tubes 118 



Kashida's litmus-lactose agar 119 



Remy 's synthetic medium 121 



Eisner's potato medium 122 



Coconut absorbent-organ cylinders 122 



Coconut absorbent-organ plates 123 



Coconut-meat cylinders 123 



Coconut leafstalk-tissue plates 123 



Coconut-water cultures. 123 



Coconut-oil media 123 



228 



