42 



HISTORY AND CAUSE OF THE COCONUT BUD-EOT. 



was not used or the tissue at the bases of the leaves was too hard to 

 be easily infected. 



The following November a new series of inoculations was made in 

 the same grove, near Baracoa, Cuba. Cultures were obtained from 

 four different trees, and inoculations were made into 13 others. 

 Just as in previous isolations of organisms from diseased material, 

 two types of colonies had predominated — the round and the irregular, 

 both of them white — so in the isolations from this series these two 

 types predominated and both of them were used for the inoculations. 

 The results are shown in Table II. 



Table II. — Inoculations of coconut trees, November 2 andS, 1908. 



Table II, in contrast to Table I, shows that many of these inocula- 

 tions were successful. Full descriptions omitted from the table are 

 here given: 



No. 337 was examined 8 days after inoculation, when it showed rot just below 

 the heart (PI. VIII) and along the inoculation hole and adjacent tissues. The central 

 leaves of this tree also had a wet rot considerably higher than the inoculation, but 

 there was no visible connection between the two. 



No. 380 was examined 11 days after inoculation and showed a soft, rotted area 

 about 2.5 centimeters in diameter, running the entire length of the inoculation hole. 

 This rot was the typical soft, white rot. The strainer was browned and rotted over a 

 large area downward from the inoculation hole (PI. IX, fig. 1). 



No. 417 was examined after 8 days and showed only a slight rot, a water-soaked 

 area about 3 centimeters in diameter, which extended from the inoculation hole; 

 but it did not show the typical soft, white decay of the tissues. The middle leaves 

 were also diseased higher up and apparently independent of the inoculation. 



No. 421 was examined after 9 days, and showed an area about the hole very 

 distinctly water soaked and discolored. There was clearly a soft rot in the tissues 

 below the heart not definitely connected with the inoculation. 

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