84 



HISTORY AND CAUSE OF THE COCONUT BUD-EOT. 



Table XV. — Growth of coconut cultures Nos. 1 to 6 and Bacillus coli on MacConkey's 

 bile-salt agar with neutral red in slant tubes, April 22 to May 2, 1910, at 37° C. 



Table XVI. — Growth of coconut cultures Nos. 1 to 6 and Bacillus coli on MacConkey's 

 bile-salt agar with neutral red on plates, April 26 to May 2, 1910, at 37° C. 



Culture. 



2 days. 



4 days. 



6 days. 



14 days. 



4 



5 

 6 



Bacillus 

 coli. 



Numerous fairly large 

 white colonies. 



Many tiny submerged 

 bright pink colonies; 

 surface colonies 

 small, round, white 

 or with pinkish 

 tinge. 



Few colonies with pe- 

 culiar tiny projec- 

 tions. 



Few colonies; not at all 



characteristic. 



Same as culture 2 



Few colonies; not at all 



characteristic. 



Round, wet-shining, 

 semitransparent, 

 slightly pinkish col- 

 onies; agar translu- 

 cent. 



Numerous tiny sub- 

 merged pink colo- 

 nies; moderate num- 

 ber of surface white 

 or pinkish colonies; 

 agar dull pink, 

 opaque. 



Numerous tiny sub- 

 merged pink colo- 

 nies; moderate num- 

 ber of surface white 

 or pinkish colonies; 

 agar translucent. 



Same as culture 3 



Same as on fourth 

 day. 



.do. 



.do. 



Like culture 2. 



Same as culture 2 



Like culture 5, only 

 agar is translucent. 



Like culture 5. 



.do. 



.do. 

 .do. 



.do. 



Same as on sixth 

 day. 



Pink surface colonies; 

 bright pink sub- 

 merged colonies; 

 agar semiopaque. 



Same as culture 2, 

 only agar is translu- 

 cent. 



Do. 



Like culture 2. 



Pink surface colonies 

 bright pink sub- 

 merged colonies; 

 agar translucent. 



Like culture 2. 



From these experiments it will be seen that the organism in question 

 grew very well on this medium, equally well with the Bacillus coli used. 

 There was a little variation in the plates, but all the tubes were prac- 

 tically alike with the exception of No. 1. It will be noted that the 

 greenish-yellow fluorescence was only a transitory character, and that 

 subseciuent to it a bright pink or slightly purplish-pink semiopaque 

 color was produced c[uite in contrast to the semitransparent orange- 

 red of the check tubes. This reaction appears to be similar to that 

 already discussed in the foregoing pages. 



228 



