86 HISTORY AND CAUSE OF THE COCONUT BUD-EOT. 



tests with check solutions resulted in four drops of the sugar solution 

 being sufficient to reduce completely 1 c. c. of Fehling's solution. 

 The cultures gave results as follows: 



No. 2: 2 drops reduced 1 c. c. of Fehling's; that is, twice the amount of sugar. 



(This must be incorrect.) 

 No. 5: 4 drops reduced 1 c. c. of Fehling's; that is, no measurable amount of 



sugar was consumed. 

 No. 6: 5 drops reduced 1 c. c. of Fehling's; that is, one-fifth the amount of sugar. 

 Bacillus coli: 6 drops reduced 1 c. c. of Fehling's; that is, one-third the amount 



of sugar. 

 No. 3: 5 drops reduced 1 c. c. of Fehling's; that is, one-fifth the amount of sugar. 

 No. 4: 5 drops reduced 1 c. c. of Fehling's; that is, one-fifth the amount of sugar. 



(2) Cultures in medium 4268 tested after five days in Dunham's 

 solution with 1 per cent dextrose, incubated at 37° C. 



Bacillus coli: 10 c. c. reduced 16.5 c.c. Fehling's = one-fifth of the total 



amount. 

 Bacillus coli a: 10 c. c. reduced 18 c. c. Fehling's = one-tenth of the total 



amount. 

 No. 5: 10 c. c. reduced 17 c. c. Fehling's = three-twentieths of the total amount. 

 No. 5 a: 10 c. c. reduced 18 c. c. Fehling's = one-tenth of the total amount. 



According to calculation the check tube of 10 c. c. with 1 per cent 

 dextrose requires 20 c. c. of Fehling's to reduce it. 



(3) Cultures similar to those in experiment 2 tested after 47 days. 

 These cultures were dried down to less than one-half then- original 



amount. As only a portion of each tube was tested the cultures were 

 diluted to their original amount and well shaken up before using. It 

 was found by repeated experiments with tubes of Bacillus coli and 

 coconut No. 5 that 1 c. c. of the culture solution just completely 

 reduced 1 c. c. of Fehling's solution. As 1 c. c. of Fehhng's is supposed 

 to reduce 0.05 grams of dextrose, 1 c. c. must reduce 0.005 grams, and 

 as the amount of dextrose used was 0.01 grams to the cubic centi- 

 meter, one-half the original amount had been consumed by the 

 bacteria. 



(4) Cultures in medium 4193, 1 per cent peptone and 2 per cent 

 dextrose, tested after 12 days. 



Ten c. c. of culture No. 5 in this solution were able to reduce only 

 30 c. c. Fehling's, thus showing that about one-fourth of the sugar had 

 been used. 



It required 40 c. c. of Fehling's solution to be completely reduced 

 by the 10 c. c. of the check culture solution. 



(5) Cultures of February 26 in medium 4192, tested after 23 dajrs. 

 (See experunent 1.) The number of cubic centimeters of Fehling's 

 solution reduced by 10 centimeters of culture was as follows: 



No. 5, SS; Bacillus coli, 20; No. 1, 15; No. 3, 30; No. 6, 32; No. 4, 38; 

 check, 43; check, 43. 



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