112 



HISTORY AND CAUSE OF THE COCONUT BUD-EOT. 



of the culture solutions. In solutions containing as high as 3 per 

 cent NaCl a good indol reaction was obtained, the color corresponding 

 to rose-violet tint No. 1, Repertoire de Couleurs, in the light tubes 

 and darker in others. 



Table XXVII. — Growth of coconut cultures Nos. 1 to 6 and Bacillus coli in Dunham's 

 solution ivith varying amounts of sodium, chlorid, at 22° C, as shown in experiments 

 1, 2, and 3, February 8 to March 14, 1910} 



Medium. 



Dunham's solu- 

 tion— 

 With 1 per 

 cent NaCl 

 (4137). 



With 1.5 per 

 centNaCl 

 (4138). 



With 2 per 

 cent NaCl 

 (4139). 



With 2.5 per 

 cent NaCl 

 (4140). 



With 3 per 

 cent NaCI 

 (4141). 



With 4 per 

 cent NaCl 

 (4159). 



With 5 per 

 cent NaCl 

 (4160). 



With 6 per 

 cent NaCl 

 (41G1). 



With 7 per 

 cent NaCl 

 (4162). 



1 day, experi- 

 ments 1 and 3. 



3 days, experi- 

 ment 2. 



5 days, experi- 

 ment 1. 



All moderately 

 clouded ex- 

 cept No. 4, 

 which is thin 

 with floccu- 

 lence. 



All moderately 

 clouded e x - 

 cept No. 4, 

 which has a 

 flocculent sus- 

 pension. 



In experiment 1 

 same as with 1 

 per cent; in 

 experiment 3 

 all are thinly 

 clouded. 



A trifle less 

 clouded than 

 with 1 per 

 cent. 



All are thinly 

 clouded. 



All are thinly 

 clouded. B. 

 coli is a trifle 

 thinner than 

 the rest. 



Nos. 1 and 5 are 

 t li i n ; others 

 are practically 

 clear. 



All clear except 

 No. 1, which is 

 very thin. 



All are clear. 



All thinly 

 clouded; 

 very thin 

 films. 



.do. 



All thinly 

 clouded; 

 bf»rely per- 

 ce p t i b 1 e 

 films. 



All thinly 

 clouded. 



Moderate 

 growth; small 

 precipitate; 

 thin films. 



Moderately 

 clouded; No. 1 

 has flocculent 

 suspension; all 

 have thin 

 films. 



All but B. coli 

 have thin 

 films; all mod- 

 erately cloud- 

 ed. 



.do. 



All moderately 

 clouded; thin 

 films. 



7 days, experi- 

 ments 1 and 2. 



8 days, experi- 

 ment 3. 



No. 5 tit rated -t- 

 2: check titrat- 

 ed+4. 



No. 5 titrated -I- 

 3; check titrat- 

 ed -1-4.5. 



No. 5 titrated -f 

 4:checktitrat- 

 ed-l-5. 



All thinly cloud- 

 ed; small pre- 

 cipitate. 



All thinly cloud- 

 ed; small pre- 

 cipitate. 



Nos. 1,3, 4, and 

 6 thinly cloud- 

 ed and small 

 amount of pre- 

 cipitate; Nos. 

 2 and 5 barely 

 clouded; B. 

 coli appears 

 clear. 



All moderately 

 clouded; mod- 

 erate precipi- 

 tate; no films; 

 Nos. 1 and 2 

 begin to clear. 



All moderately 

 clouded and 

 moderate pre- 

 cipitate; Nos. 

 2 and 3 have 

 thin films. 



All well clouded 

 with moder- 

 ate precipi- 

 tate; Nos. 2 

 and 4 have 

 thin films. 



All well cloud- 

 ed; small pre- 

 cipitate; No. 2 

 is beginning 

 to clear; no 

 films. 



All thinly cloud- 

 ed; small pre- 

 cipitate. 



.\11 thinly cloud- 

 ed; small pre- 

 cipitate; no 

 films. 



Nos. 1, 3, and 4 

 are thinly 

 clouded; 

 small precipi- 

 tate. 



Nos. 1, 3, and 4 

 thinly clouded 

 small precipi- 

 tate; others 

 barely cloud- 

 ed, with very 

 small precipi- 

 tates. 



1 Experiment 1, Feb. 8 to Feb. 25; 2 and 3, Feb. 17 to Mar. 14. 



228 



