LABORATOEY AND GREENHOUSE STUDIES. 



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Table XXVIII. — Growth and production of gas (in mm.) in peptone with levulose, 

 galactose, and mannit in fermentation tubes, February 21 to March 8, 1910, at 22° C. 



• Well clouded in both ends; a few bubbles of gas. 



2 Well clouded in open end; thin in closed end. 



3 Well clouded in both ends. 



^ Moderately clouded in both ends. 



i> Moderately clouded in both ends; three small bubbles of gas. 



6 Well clouded in both ends; one large bubble of gas. 



' AVell clouded in open end; moderate in closed end; many small l)ubbles of gas. 



8 Well clouded in both ends; a few large bubbles of gas. 



9 Well clouded in both ends; many small bubbles of gas. 



kashida's litmus-lactose agar. 



Kashida's medium ^ consists of bouillon containing 1 .5 per cent of 

 agar, 2 per cent of milk sugar, 1 per cent urea, and 3 per cent of 

 tincture of litmus. The culture medium should be blue. Wlien 

 liquefied, inoculated with the colon bacillus, poured into petri dishes, 

 and allowed to stand 16 to 18 hours in the incubator, the blue color 

 passes off and the culture medium becomes red. If a glass rod 

 dipped in HCl be held over the dish, vapor of ammonium clalorid 

 is said to be given off. The typhoid bacillus produces no acid in 

 this medium, and there is consequently no change in color. 



Table XXIX. — Growth of coconut cultures Nos. 1 to 6 and Bacillus coli on plates of 

 Kashida's medium, April 14 to IS, 1910, at 22° C. 



A glass rod dipped into hydroclxloric acid which was not fuming 

 was held over each of the plates, and in the cases of cultures 1 and 2, 

 which were still blue, white fumes arose from the hanging drop. 

 In none of the other plates, aU of which were entirely red or partly 



1 MacFarland, Joseph. Textbook upon the Pathogenic Bacteria, p. 487. 



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