LABORATORY AND- GREENHOUSE STUDIES. 123 



COCONUT ABSORBENT-ORGAN PLATES. 



Pieces of the absorbent oi<^aii were sterilized by means of alcohol, 

 mercuric chlorid, and distilled water, and then placed in petri dishes. 

 The plates were then inoculated, but even after eight days there ap- 

 peared to be no growth on the tissues. 



COCONUT-MEAT CYLINDERS. 



Cylinders were made in the usual way from coconut meat and placed 

 in test tubes with enough water to cover the lower half of each cylin- 

 der. The tubes were sterilized on three successive days by steam- 

 ing and were then inoculated. The growth resulted as follows: 



After 6 days: The submerged parts of the cylinders were pink in the culture tubes 

 and white in the check tubes. The top of the cylinder was dark and translucent. 

 The liquid was moderately clouded. 



After 27 days: All the checks were pink under water; coconut Nos. 2 and 5, and 

 Bacillus coll (Hitchings, XIV, and VI-ll-V-09) were greenish white under 

 water, and the water was of the same color; the others, including Bacillus coli 

 (B. A. I.) were dark pink under water; no definite film in any case; the growth 

 appears to have been only moderate. 



COCONUT LEAFSTALK-TISSUE PLATES. 



Large pieces of leafstalk of both old and very young leaves were 

 sterilized in alcohol, mercuric chlorid, and distilled water and then 

 placed m plates and inoculated. 



After 12 days: Bacillus coli (Hitchiugs) on a large hard piece of leafstalk, surface 

 mottled, but no rot. 



Bacillus coli (VI-ll-V-09) on a very fibrous piece of leafstalk, covered with a 

 brownish slimy mass; not soft rotted to any extent. 



Bacillus coli (XIV) on rather young leaf tissues, black, soft rotted as in similar 

 portions of a naturally infected tree ; exactly the appearance of the leaf-base 

 rot in the mature tree. 



Bacillus coli (B. A. I.) on two slender leaflets became completely dried up. 



Coconut 5, one of the pieces of young tissue was black, soft rotted as in typi- 

 cal cases. 



No other coconut organism was tried. 



COCONUT-WATER CULTURES. 



The ordinar}^ water from the ripe coconut was sterilized m tubes 

 and inoculated. All of the tubes became moderately clouded in 

 two da3^s at 37° C, but they produced no gas and did not remain 

 clouded long. In 15 days all were practically clean except coconut 

 No. 1, which was well clouded. 



COCONUT-OIL MEDIA. 



Coconut oil was pressed out of finely cut coconut meat both before 

 and after cooking, and this was purified by mixing with alcohol and 

 then drying out completely. After purification it was a perfectly 



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