BACILLUS COLI, THE CAUSE OF BUD-ROT. 



137 



The proof of tlio cause of the bud-rot will depend for its verity 

 upon the similarity of the various cultures isolated from diseased 

 tissues and upon the constancy of the reactions. Dissimilarity or 

 variation will require satisfactory explanation, or it will count 

 against the statement to be proved. For the i)roof, so far as inocu- 

 lations are concerned, the results cited seem sufficient. There can 

 be no question tliat good infections were obtained. If now the simi- 

 larity of the organism injected into the tissues and of the organism 

 isolated from the tissues in various experiments be shown, the cause 

 of bud-rot, and, moreover, Bacillus coli as the cause, will be demon- 

 strated. In order to show briefly and in a concise form the similari- 

 ties and differences among these organisms as ascertained in the 

 cultural work, Table XXXI has been prepared. 



Table XXXI. — Summary of characters^ of the coconut organisms ajid of Bacillus coli. 



Detailed features. 



Morphology of organism 



Morphology of colony on agar plate, agar stab, streak, 



gelatin stab, plate 



Facultative anaerobism 



Gelatin liquefaction 2 



Acid in dextrose 



Gas in dextrose 



Acid in lactose 



Gas in lactose 



Acid in saccharose , 



Gas in saccharose 



Reduction of nitrates 



Pigment production 



Growth on starch media 



Acid in glycerin 



Gas in glycerin 



Dolt's litmus-lactose-glycerin agar s 



Neutral red: 



With peptone water + dextrose in fermentation 

 tubes 



With dextrose in agar ^ 



With lactose in agar 



With saccharose in agar 



With glycerin in agar 



Without sugar in agar 



MacConkey 's bile-salt agar with neutral red: ^ 



In tubes 



In plates ■• 



Test 1 of D. Rivas 



Test 3 of D. Rivas 



Endo's fuchsin agar '■' 



Stoddart's plate medium 



Hiss's tube medium 



Sterile milk 



Litmus milk 



Production of— 



Indole 



Phenol ' 



H2S 



Ammonia 



Coconut culture. 



-I- 



+ 

 + 



± 

 + 



+ 



+ 



+ 

 + 

 + 



-H 



-I- 

 -I- 

 -f 

 + 



3. 



+ 



+ 

 + 



± 

 + 

 + 

 + 



+ 



+ 

 + 



+ 

 + 

 + 

 + 

 + 

 + 

 + 



+ 



+ 

 + 



+ 



+ 



+ 



+ 

 ± 

 + 

 ± 

 + 

 ± 

 + 



± 

 -t- 



+ 



+ 

 + 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



+ 



+ 



+ 



+ 

 ± 

 + 

 ± 

 + 

 ± 

 + 



± 

 + 



+ 



+ 



+ 



+ 



+ 

 + 

 + 

 + 



+ 

 + 



+ 

 + 



+ 



+ 

 + 



-I- 

 -I- 

 -t- 



± 

 + 

 + 



+ 

 + 

 + 

 + 

 + 

 + 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



+ 



+ 

 + 



BaciDus 

 coli. 



1 Explanation of arbitrary signs used in the table: + indicates that the reaction is positive, or merely 

 that the characters are all the same; — indicates that the reaction is negative; ± indicates that the reaction 

 sometimes occurs and sometimes does not; -H indicates that the reaction is a variation in the positive reac- 

 tion, which is the same in all cultures so marked; ±1 indicates that there is a variation in the positive reac- 

 tions which sometimes occurs and sometimes does not. 



2 For variations in No. 1, see p. 66. 

 ' No. 1 tends to bleach. 



* Nos. 2 and 5 and Bacillus coli are slightly different from others. 



5 No. 3 and Bacilhis coli are slightly different from others. 



6 Bacillus coli a trifle more than others. 

 ' This result is questioned. 



228 



