IBS- 



HISTORY AND CAUSE OF THE COCONUT BUD-EOT. 



Table XXXI.— Summary of characters of the coconut organisms and of Bacillus colir— 



Continued. 



Detailed features. 



Coconut culture. 



Enzymes in milk ' , 



Growth in nitrogen-free media: 



With ammonium tartrate ^ 



With ammonium citrate ^ 



With ammonium lactate 



With asparagin 



With sodium asparaginate 



Fischer's mineral solution: 



Alone * 



With dextrose 



WithKNOa* 



With cane sugar 



With peptone 



With peptone + dextrose 



With peptone + glycerin 



With glycerin 



With cane sugar + KNO3 



With dextrose + KNO3 



Malachite green in sugar 



Caflein in agar 



Capaldi and Proskauer: 



No.l 



No. 2 



Dunham's solution: 



With 7 per cent NaCl 



With f. per cent NaCl 



Uschinsky 's solution 



Cohn's solution 6 



Potato agar 



Carrot agar 



Litmus-lactose agar 



Oxalic-acid agar (0.2 per cent acid) 



Coconut cylinders 



In fermentation tubes: 



Acid from galactose 



Gas from galactose ' 



Acid from levulose 



Gas from levulose » | -h 



Acid from mannit 



Gas from mannit 



Kashida's medium 



Temperatures (degrees Centigrade): 



Optimum 



Maximum 



Minimum 



Thermal death-point 



+ 



+ 

 -H 



35 



47 



8.5 



57 



+ 

 + 



+ 



+ 

 + 

 -H 

 + 

 + 

 + 



+ 

 -f 



-I- 

 -I- 

 -I- 



+ 

 + 

 -I- 



4- 



-I- 



+ 



35 



47 



8.5 



57 



-H 

 -H 



■H 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



+ 



-H 

 -H 



+ 



+ 



+ 

 + 



+ 



+ 



+ 

 + 

 + 

 + 

 + 



a 



35 



47 



8.5 



57 



-H 

 -H 

 -H 

 -H 

 ±1 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 4- 

 + 



-I- 

 + 



-H 



+ 

 + 

 + 



+ 

 + 



35 



47 



8.5 



57 



+ 

 + 



+ 

 + 

 ±1 



-H 

 -H 

 -H 

 -H 

 ±1 



Bacillus 

 coli. 



■» 



-t^ 



-H 



+ 

 + 



+ 



t 



T 



-I- 

 + 



-r 



-I- 

 -I- 

 + 

 + 



+ 

 + 



+ 

 + 

 + 



35 



47 



8.5 



57 



1 II present, in only very small quantities. 



2 Nos. 3, 4, and 6 and Bacillus coli have much poorer growth than others. 



3 Growth in these tubes varies considerably in amount and color of precipitate. 

 * There appears to be a very slight clouding. 



6 Very slight clouding, if any. 



» V'ery slight growth, if any," in all but 5. 



' No. 1 produced 69 mm. of gas; others ranged from 32 to 43. 



' No. 1 produced 51 mm. of gas; others averaged 14 to 20 mm. 



The importance of the variations shown in the foregoing tables 

 has been discussed under the various experiments. It will suffice to 

 summarize them briefly at this point. 



In comparing coconut cultures Nos. 4, 5, and 6 (which are inocula- 

 tion (6), isolation (5), reinoculation, and reisolation (4) cultures of 

 the same origin) with Bacillus coli it is seen that tliey are all identical 

 until it comes to acid and gas in lactose. In that medium No. 5 and 

 Bacillus coli have always behaved alike; Nos. 4 and 6 have ahvays 

 acted similarly to each other and frequently in tliis medium similarly 



228 



