COMPARISON OF BACILLUS COLI WITH VARIOUS ORGANISMS. 143 



Of fermentation tubes of April 16, 1908, with various coconut cultures, 16 out of 

 24 produced gas in amounts from 5 to 9 centimeters in seven days. 



Six bouillon tubes containing cultures from Trinidad on July 23, 1907, showed some 

 gas formation. 



Two bouillon tubes of July 12, 1907, from Demerara showed some gas formation. 



Five bouillon tubes of July 13, 1907, from Demerara showed some gas formation. 



Three tubes of July 29, 1907, from Demerara in litmus milk became bleached and 

 coagulated . 



Four tubes on August 7, 1907, from Demerara in litmus milk become reddened 

 and coagulated. 



Two litmus-milk tubes of Cuba became bleached. 



One litmus-milk tube of Jamaica became partially bleached and another of Jamaica 

 became bleached and coagulated. 



Of the cultures of 1909 isolated in 1908, two tubes of 248 Cuba 

 bleached Htmus milk and coagulated it. Of all the coconut cultures 

 in use by the ^vriter early in 1909, seven different ones blued htmus 

 milk, seven turned the litmus pink but did not coagulate the milk, 

 and 23 both turned the litmus pink and coagulated the milk. This 

 change from blue to pink in some cases j)rogressed even to bleaching. 

 Some cultures from each of the trees 380, 78, 422, 248, and 153, 

 reddened or both reddened and coagulated the litmus milk as A\dth 

 typical Bacillus coli. 



Many cultures were isolated in the fall of 1909, among which were 

 the two, 508 N (coconut No. 6) and 505 N (coconut No. 3), carried 

 through the experiments described on previous pages. 



Of this lot isolated September 23, 1909, which was designated 

 series N (Table IV, p. 45), many show the Bacillus coli reactions. 



No. 502 failed to show any reduction of nitrates. 



No. 503, two tubes, both showed good reduction of nitrates. 



No. 504 failed to show any reduction of nitrates. 



No. 505, two tubes, both showed reduction of nitrates. 



No. 506, three tubes, all showed reduction of nitrates. 



Nos. 502 and 504 failed to show the reaction in nitrate bouillon 

 that is characteristic of Bacillus coli. Several cultures, however, 

 were taken from each of the trees and designated by the same tree 

 number, but with some different tube number, and possibly represent- 

 ing different organisms. For this reason other tubes were selected 

 to repeat the nitrate test, with the following results: 



Nos. 502, 504, 505, 506, and 508 showed good reduction of nitrates. 



Transfers from these cultures were inoculated into trees, produced 

 a disease, and have been studied subsequently, as shown in the 

 foregoing pages. Other cultures were isolated from trees and, in 

 some cases, inoculated, but have not been further studied. The 

 original cultures, however, were gro\\Ti in nitrate bouillon and show 

 the following results: 



Nos. 503, 507, 601, 602, 603, and 604 show good reduction of nitrates. 

 228 



