144 HISTORY AND CAUSE OF THE COCONUT BUD-EOT. 



Thus, it is shown that some tubes of all these original cultures 

 responded to tliis test as does the colon organism. 



Cultures of these organisms were made in litmus milk and showed 

 the following results: 



Series N: Nos. 501 and 501a tiirned litmus milk blue. Nos. 502, 504, 506, and 508 

 (six tubes) reddened litmus milk. 



Series S: This series was isolated from inoculations made with Series N. No. 502 

 blued litmus milk. Nos. 504, 505, 506, and 508 reddened litmus milk. A large number 

 of tubes of 508 S were all reddened and coagulated. 



Of the series R, the isolations from inoculations with series S, 

 cultures were made on Htmus-lactose agar with 505 R (coconut Xo. 1) 

 and 508 R (coconut No. 4). Ten tubes of 505 R all show-ed reddening 

 of the agar as with Bacillus coli. Fifteen tubes of 508 R showed a 

 similar reddening, w^hile the remaining 18 tubes did not. No other 

 of the early coconut cultures were tried on this medium. 



In fermentation tubes wdth dextrose and peptone many of the 

 cultures produced gas. 



The production of gaa (in millimeters) by cultures of series N at room temperature 

 for nine days was as foUows: No. 502, 24; No. 504, 25; No. 505, 21; No. 506, 30; 

 No. 507, none; No. 508, 26; No. 601, 81; No. 602, 29; No. 603, 28; No. 603a, 31; 

 No. 604, 26. 



The production of gas by cultures of series S at room temperat^lre for nine days 

 was as follows: No. 503, 21 mm.; No. 504, none; No. 505, 24; No. 506, 64; No. 508, 

 none; No. 508a, 24. 



It must be borne in mind that each number represents several 

 tubes derived from as many colonies, and in each one of these tests 

 given only one of the tubes is represented. For example, in the pre- 

 ceding paragraph one tube of 504 failed to produce gas, while 503 

 and 505, etc., did produce gas. Other tubes of 504 might also have 

 done so if they had been tried. 



The foregoing paragraphs show that the writer had early cultures 

 isolated from diseased coconut trees and that some of these cultures 

 resembled Bacillus coli so far as tested. It must also be admitted 

 that there were many cultures w^hich gave positive evidence of not 

 being Bacillus coli. It will now be of interest to turn to Dr. Smith's 

 cultures obtained by him in 1904. 



Of tliis series the so-called coconut B responds to many of the 

 tests for Bacillus coli. In every medium in M'hich it was tested it 

 gave a positive reaction for that organism. The results for coconut B 

 are as follows: 



Reduces nitrates. 



Produces indol in Dunham's solution. 

 Produces no nitrous acid in Dunham's solution. 



Produces gas in glucose, in cane sugar, in lactose, in maltose, in glycerin, and in 

 mannit. 

 Coagulates litmus milk. 

 228 



