14 



A difference of about 6 per cent, and 18 per cent, respectively in favor of 

 'inoculation. 



Plot Experiments. Clover sown May 9th. Each plot 22 by 33 feet. 



lbs. ozs. 



Hay crop from untreated seed (2 cuttings) 46 11 



" " " inoculated seed (2 cuttings) .. .;■_ . 59 13 



A difference of a little more than 22 per cent, in favor of inoculated seed. 



Plot Experiments. Alfalfa, Sown May 9th. Each plot 22 by 33 feet. 



lbs. ozs. 



Hay crop from untreated seed (3 cuttings) 88 4 



" " " treated seed (3 cuttings) 62 13 



A difference of nearly 3o per cent, in favor of the untreated seed. 



Mr. Shutt makes no explanation of this difference in favor of the 

 untreated seed, but writes : 



"The nodules on the foots of the untreated alfalfa appeared to be 

 just as numerous as on the roots of the inoculated. 



"The soil is a light, sandy loam, fairly rich in humus. It has in all 

 probability carried legumes, but not for some years." 



Mr. C. Jarvis, of the Department of Horticulture, Cornell University, 

 tried the culture on the Mohawk variety of bean. Each row was 20 feet 

 long. His results are as follows : — 



"1. Mohawk Beans untreated — 30 plants, no nodules. 



" 2. Beans treated with fresh culture and sown at once — 27 plants, 

 12 with nodules. 



"3. Beans seeded two weeks later but treated same time as in 

 Experiment 2. — 35 plants, 2 with nodules. 



"4. Beans untreated sown at same time as in Row 3 — 37 plants, 7 

 with nodules. . 



"5. Beans treated with culture 1 month old and sown in a moist 

 condition — 31 plants, 6 with nodules. 



" G Beans untreated sown at same time as No. 5 — 35 plants, 3 with 

 nodules." 



As will be seen from above the best results were obtained from fresh 

 culture used according to directions ; the remaining experiments were 

 conducted on Mr. Jarvis' own initiative. Mr. Jarvis remarks: "Had 

 some other variety of bean been used in place of Mohawk, which is a very 

 hardy, strong growing variety, a difference in growth may have been 

 apparent." 



As the Bacteriological Department intends to continue experimental 

 work along this line, and will send out nitro-cultures in the spiring of 1906, 

 some information is needed regarding the use of the cultures and their 

 application; hence a few notes are given for the guidance ojf those who 

 desire to experiment with these beneficial bacteria. 



