54 



dioxide content in the eg-gf chamber was decreased from 10.46 parts for 

 water only to 8.29 parts for water and zenoleum, and from 9.15 parts in 

 the dry machine to 5.86 parts in the dry machine disinfected with zen- 

 oleum. In every instance disinfecting with zenoleum resulted in a de- 

 crease of carbon dioxide, yet the use of zenoleum never failed to give a 

 better hatch, and higher vitality. While a high carbon dioxide content 

 seems decidedly beneficial in the case of machines supplied with moisture 

 only, yet it is just possible that the function it performs in artificial incu- 

 bation may be fulfilled by something else, as the results from the use of 

 buttermilk and zenoleum seem to indicate. 



Although the work on carbon dioxide is not conclusive, the results 

 so far furnish much valuable data, and establish many useful relation- 

 ships. Just what function, if any, carbon dioxide performs in incubation, 

 and to what extent it is essential, is a point on which we have not at 

 present sufficient experimental data to warrant conclusions. 



Table XX. Carbon Dioxide under Sitting Hens. 



20th day. 



6a bull. 163 



